Bacterial Recruitment to Carnivorous Pitcher Plant Communities: Identifying Sources Influencing Plant Microbiome Composition and Function.
Bacterial Recruitment to Carnivorous Pitcher Plant Communities: Identifying Sources Influencing Plant Microbiome Composition and Function.
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DOI：
10.3389/fmicb.2022.791079
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发表时间：
2022
影响因子：
5.2
通讯作者：
Young EB
中科院分区：
文献类型：
--
作者：
Grothjan JJ;Young EB
影响不同细菌向植物微生物组募集的过程仍然知之甚少。在食肉猪笼草模型系统中，单个猪笼草打开收集雨水，无脊椎动物和各种微生物群落，这种碎屑食物网由捕获的昆虫猎物维持。这项研究探讨了潜在的细菌来源如何影响投手内细菌群落的发展，宿主植物组织如何影响群落的发展，以及建立与组装群落的差异。在一个受控的温室实验中，七个重复的投手被分配到五个处理，以排除特定的细菌源或宿主组织：milliQ水只，milliQ +昆虫猎物，雨水+猎物，建立社区+猎物，人工投手与milliQ +猎物。利用细菌基因测序和功能预测，测量细胞丰度，水解酶活性和营养转化，在8-40周内检查了群落组成和功能。人工和真实的猪笼草群落组成和功能的差异证实了寄主植物组织对群落发育的重要影响，但也表明这可能与寄主营养吸收有关。从空气中的细菌投手显着招聘是显而易见的，从许多类群共同的所有治疗，重叠的组合物之间的milliQ，milliQ +猎物，雨水+猎物的治疗，和一些类群独特的milliQ只有投手。社区功能测定水解酶（几丁质酶，蛋白酶）活性的昆虫猎物的影响力很大，并与昆虫营养物质迅速转化为溶解和无机源。在处理内的7个重复投手中的6个中发现细菌分类群，“核心微生物组”在8周内显示出比所有分类群更紧密的演替轨迹。建立猪笼草群落组成更稳定，超过8周，这表明了多样性的稳定性关系和微无脊椎动物对细菌的影响。这项研究广泛地表明，宿主猪笼草中的细菌组成与随机和特定的细菌招募有关，宿主植物通过捕获昆虫猎物影响微生物选择并支持微生物组。
Processes influencing recruitment of diverse bacteria to plant microbiomes remain poorly understood. In the carnivorous pitcher plant Sarracenia purpurea model system, individual pitchers open to collect rainwater, invertebrates and a diverse microbial community, and this detrital food web is sustained by captured insect prey. This study examined how potential sources of bacteria affect the development of the bacterial community within pitchers, how the host plant tissue affects community development and how established vs. assembling communities differ. In a controlled greenhouse experiment, seven replicate pitchers were allocated to five treatments to exclude specific bacterial sources or host tissue: milliQ water only, milliQ + insect prey, rainwater + prey, established communities + prey, artificial pitchers with milliQ + prey. Community composition and functions were examined over 8–40 weeks using bacterial gene sequencing and functional predictions, measurements of cell abundance, hydrolytic enzyme activity and nutrient transformations. Distinct community composition and functional differences between artificial and real pitchers confirm an important influence of host plant tissue on community development, but also suggest this could be partially related to host nutrient uptake. Significant recruitment of bacteria to pitchers from air was evident from many taxa common to all treatments, overlap in composition between milliQ, milliQ + prey, and rainwater + prey treatments, and few taxa unique to milliQ only pitchers. Community functions measured as hydrolytic enzyme (chitinase, protease) activity suggested a strong influence of insect prey additions and were linked to rapid transformation of insect nutrients into dissolved and inorganic sources. Bacterial taxa found in 6 of 7 replicate pitchers within treatments, the “core microbiome” showed tighter successional trajectories over 8 weeks than all taxa. Established pitcher community composition was more stable over 8 weeks, suggesting a diversity-stability relationship and effect of microinvertebrates on bacteria. This study broadly demonstrates that bacterial composition in host pitcher plants is related to both stochastic and specific bacterial recruitment and host plants influence microbial selection and support microbiomes through capture of insect prey.
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影响因子：
0.6
作者：
FISH, D;HALL, DW
通讯作者：
HALL, DW
影响因子：
4.2
作者：
Allali I;Arnold JW;Roach J;Cadenas MB;Butz N;Hassan HM;Koci M;Ballou A;Mendoza M;Ali R;Azcarate-Peril MA
通讯作者：
Azcarate-Peril MA
影响因子：
0.6
作者：
CRESSWELL, JE
通讯作者：
CRESSWELL, JE
紫色的投手植物（sarracenia rosea）死于沿海投手植物沼泽实验性风暴潮后的倒退和部分社区拆卸。
影响因子：
3.7
作者：
Abbott MJ;Battaglia LL
通讯作者：
Battaglia LL
影响因子：
1.5
作者：
Bittleston, Leonora S.;Baker, Christopher C. M.;Pierce, Naomi E.
通讯作者：
Pierce, Naomi E.