Isolation and characterization of cDNAs from Lycopersicon esculentum and Arabidopsis thaliana encoding the 33 kDa protein of the photosystem II-associated oxygen-evolving complex.
Isolation and characterization of cDNAs from Lycopersicon esculentum and Arabidopsis thaliana encoding the 33 kDa protein of the photosystem II-associated oxygen-evolving complex.
复制标题
番茄和拟南芥中编码与光系统 II 相关的释氧复合物的 33 kDa 蛋白质的 cDNA 的分离和表征。
DOI：
10.1007/bf00018562
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发表时间：
1990
影响因子：
5.1
通讯作者：
Cashmore,AR
中科院分区：
文献类型：
--
作者：
Ko,K;Granell,A;Bennett,J;Cashmore,AR
We have identified and isolated cDNA clones of the 33 kDa protein of the oxygen-evolving complex (OEE1) fromLycopersicon esculentum(tomato) andArabidopsis thalianaand determined their nucleotide sequences. The cDNA clones and antibodies prepared against OEE1 were used as probes to examine the expression of theoee1gene with respect to regulation by light, organ specificity, and ripening stage of tomato fruit. The steady-state mRNA level is regulated by light, being present in light-grown plants and absent in etiolated seedlings. Theoee1transcripts that accumulate during growth in the light were reduced to non-detectable or low levels by a 3-day dark treatment. Theoee1gene also exhibits differential expression in various organs of the tomato plant. Steady-state mRNA level was highest in immature leaves and absent in roots while the protein was most abundant in mature leaves and absent in roots. During tomato fruit ripeningoee1mRNA decreases to a low level in the pericarp while the protein level decreases below detection. The expression ofoee1appears to be under the control of a complex mechanism regulating both the amount of RNA and its translation.
