The endometrium responds differently to cloned versus fertilized embryos
The endometrium responds differently to cloned versus fertilized embryos
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DOI：
10.1073/pnas.0811841106
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发表时间：
2009-04-07
影响因子：
11.1
通讯作者：
Wolf, Eckhard
中科院分区：
文献类型：
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作者：
Bauersachs, Stefan;Ulbrich, Susanne E.;Wolf, Eckhard
虽然体细胞核移植（SCNT）克隆在牛中比迄今为止测试的任何其他物种都更有效，但妊娠失败率很高，这与胎盘的结构和功能异常有关。我们检验了这一假设，即这些变化可能源于胚胎-母体在围着床期的相互作用。因此，我们评估了子宫内膜对SCNT胚胎（由7种不同的胎儿成纤维细胞系产生）的反应，并与体外受精（IVF）产生的胚胎进行了比较。将SCNT胚胎和IVF胚胎在相同条件下培养至囊胚期（第7天），并转移至相应的受体，在妊娠第18天屠宰。使用定制的cDNA微阵列富集在动情周期和/或早期妊娠期间子宫内膜和/或输卵管上皮中差异表达的转录物，获得子宫内膜样品的mRNA谱。总体而言，SCNT组的mRNA谱变化大于IVF组。此外，58个转录本在SCNT和IVF妊娠的子宫内膜中差异丰富。突出的例子是孤儿核受体COUP-TFII和连接蛋白43，两者已知在子宫容受性和孕体胎盘形成中起重要作用。这些研究结果表明，牛克隆妊娠中的胎盘衰竭可能起源于围着床期发育的异常胚胎-母体通讯。子宫内膜转录组谱可以作为评估SCNT胚胎建立妊娠和发育功能性胎盘的能力的工具。
Although somatic cell nuclear transfer (SCNT) cloning is more efficient in cattle than in any other species tested so far, there is a high rate of pregnancy failure that has been linked to structural and functional abnormalities of the placenta. We tested the hypothesis that these changes may originate from disturbed embryo-maternal interactions in the peri-implantation period. Therefore, we evaluated the response of the endometrium to SCNT embryos (produced from 7 different fetal fibroblast cell lines) as compared with embryos derived from in vitro fertilization (IVF). SCNT embryos and IVF embryos were cultured under identical conditions to the blastocyst stage (day 7) and were transferred to corresponding recipients, which were slaughtered at day 18 of pregnancy. The mRNA profiles of endometrium samples were obtained using a custom cDNA microarray enriched for transcripts differentially expressed in the endometrium and/or oviduct epithelium during the estrous cycle and/or early pregnancy. Overall, the variation in mRNA profiles was greater in the SCNT group than in the IVF group. Furthermore, 58 transcripts were differentially abundant in endometria from SCNT and IVF pregnancies. Prominent examples are orphan nuclear receptor COUP-TFII and connexin 43, both known to play important roles in uterine receptivity and conceptus placentation. These findings suggest that placental failure in bovine clone pregnancies may originate from abnormal embryo-maternal communication that develops during the peri-implantation period. Endometrium transcriptome profiles may serve as a tool to evaluate SCNT embryos for their ability to establish pregnancy and develop a functional placenta.