Glycerol-3-Phosphate Acyltransferase 6 (GPAT6) Is Important for Tapetum Development in Arabidopsis and Plays Multiple Roles in Plant Fertility
Glycerol-3-Phosphate Acyltransferase 6 (GPAT6) Is Important for Tapetum Development in Arabidopsis and Plays Multiple Roles in Plant Fertility
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DOI：
10.1093/mp/ssr057
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发表时间：
2012-01-01
期刊：
影响因子：
27.5
通讯作者：
Yang, Zhong-Nan
中科院分区：
文献类型：
--
作者：
Li, Xiao-Chuan;Zhu, Jun;Yang, Zhong-Nan
甘油-3-磷酸酰基转移酶（GPAT）介导形成甘油脂的初始合成步骤，甘油脂充当生物膜的主要组分和能量的主要储存形式。GPAT 6是拟南芥GPAT家族的成员，其在萼片和花瓣中的角质生物合成中至关重要。在这项工作中，GPAT 6在花药发育和植物育性中的功能进行了分析。在花药发育过程中，GPAT 6在绒毡层和小孢子中高表达。基因敲除突变体gpat 6导致种子产量大幅下降。本研究表明，GPAT 6基因的缺失导致绒毡层发育缺陷，绒毡层中内质网（ER）分布减少，这在很大程度上导致花粉粒败育和花粉壁形成缺陷。此外，花粉萌发和花粉管伸长也受到影响。双突变体分析表明，GPAT 6和GPAT 1对小孢子从四分体中释放和雄蕊花丝伸长有协同作用。这项工作表明GPAT 6在拟南芥雄蕊发育和育性中起着多重作用。
Glycerol-3-phosphate acyltransferase (GPAT) mediates the initial synthetic step for the formation of glycerolipids, which act as the major components of biological membranes and the principal stored forms of energy. GPAT6 is a member of the Arabidopsis GPAT family, which is crucial for cutin biosynthesis in sepals and petals. In this work, a functional analysis of GPAT6 in anther development and plant fertility was performed. GPAT6 was highly expressed in the tapetum and microspores during anther development. The knockout mutant, gpat6, caused a massive reduction in seed production. This report shows that the ablation of GPAT6 caused defective tapetum development with reduced endoplasmic reticulum (ER) profiles in the tapetum, which largely led to the abortion of pollen grains and defective pollen wall formation. In addition, pollen germination and pollen tube elongation were affected in the mutant plants. Furthermore, the double mutant analysis showed that GPAT6 and GPAT1 make joint effects on the release of microspores from tetrads and stamen filament elongation. This work shows that GPAT6 plays multiple roles in stamen development and fertility in Arabidopsis.