Integrated Proteomics and Transcriptomics Analyses Reveals the Possible Antifungal Mechanism of an Indoloquinoline Alkaloid Neocryptolepine against Rhizoctonia solani
Integrated Proteomics and Transcriptomics Analyses Reveals the Possible Antifungal Mechanism of an Indoloquinoline Alkaloid Neocryptolepine against Rhizoctonia solani
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蛋白质组学和转录组学综合分析揭示了吲哚喹啉生物碱 Neocryptolepine 对抗立枯丝核菌的可能抗真菌机制
DOI：
10.1021/acs.jafc.1c01385
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发表时间：
2021-06-02
影响因子：
6.1
通讯作者：
Zhang, Ji-Yu
中科院分区：
文献类型：
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作者：
Shang, Xiao-Fei;Dai, Li-Xia;Zhang, Ji-Yu
立枯丝核菌引起严重的植物病害。新隐草平对红曲霉有明显的抑菌活性。solani，但其作用方式尚不清楚。本文研究了新隐草平对红曲霉的潜在作用方式。solani整合了蛋白质组学和转录组学。结果表明，经新隐草碱处理后，红曲霉菌差异表达蛋白1012个，差异表达基因10920个。solani，大多数富集在线粒体呼吸链中。影响氧化磷酸化，导致活性氧的富集和基质金属蛋白酶的降低，并抑制复合物Ⅲ的活性，在10 μ g/mL时抑制率为63.51%。线粒体结构和功能受损。细胞色素b-c1复合物亚基Rieske（UQCRFS 1）与新隐平的结合得分高，被认为是一个潜在的目标。此外，它抑制菌核形成，并通过以浓度依赖性方式减小马铃薯伤口的直径来呈现抗真菌效果。以上结果表明，新隐草平通过与UQCRFS 1结合抑制复合物Ⅲ的活性，阻断离子传递，从而导致R.茄属菌丝体本研究为新隐草平作为替代生物杀菌剂的进一步开发奠定了基础。
Rhizoctonia solani causes serious plant diseases. Neocryptolepine presented the significant antifungal activity against R. solani, however the mode of action is unclear. In this paper, we investigated the potential mode of action of neocryptolepine against R. solani integrated the proteomics and transcriptomics. Results showed that after treatment with neocryptolepine, 1012 differentially expressed proteins and 10 920 differentially expressed genes of R. solani were found, most of them were enriched in mitochondrial respiratory chain. It affected oxidative phosphorylation led to the enrichment of ROS and the decrease of MMP, and inhibited complex III activity with the inhibition rate of 63.51% at 10 mu g/mL. The mitochondrial structural and function were damaged. Cytochrome b-c1 complex subunit Rieske (UQCRFS1) with the high binding score to neocryptolepine was found as a potential target. In addition, it inhibited the sclerotia formation and presented antifungal efficacy by decreasing the diameter of a wound in potato in a concentration-dependent manner. Above results indicated that neocryptolepine inhibited the complex III activity by binding UQCRFS1 and blocked the ion transfer to cause the death of R. solani mycelia. This study laid the foundation for the future development of neocryptolepine as an alternative biofungicide.