Co-expression network analysis identified KIF2C in association with progression and prognosis in lung adenocarcinoma
Co-expression network analysis identified KIF2C in association with progression and prognosis in lung adenocarcinoma
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DOI：
10.3233/cbm-181512
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发表时间：
2019-01-01
影响因子：
3.1
通讯作者：
Tang, Hexiao
中科院分区：
文献类型：
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作者：
Bai, Yuquan;Xiong, Lecai;Tang, Hexiao
肺癌是一种发病率和死亡率高的恶性肿瘤，其中80%为非小细胞肺癌（NSCLC）。而肺腺癌（LUAD）是NSCLC中最重要、最常见的亚型。本研究对含有LUAD （n = 226）和正常肺组织（n = 20）的微阵列数据GSE31210进行分析，鉴定出965个差异表达基因，并对其进行加权基因共表达网络分析。最后，证实了brown模块与LUAD分期之间存在显著的相关性。在显著性模块中，共鉴定出54个网络枢纽基因，其中6个也被鉴定为蛋白质-蛋白质相互作用网络的枢纽基因。验证中，KIF2C与疾病分期的相关性高于其他枢纽基因（p < 0.001, R2 = 0.955）。功能富集表明KIF2C与细胞有丝分裂和细胞周期有关。结合临床病理参数，我们发现KIF2C的高表达与LUAD的复发和肿瘤分期密切相关。生存分析显示，KIF2C高表达的LUAD患者的总生存率显著降低。基因集富集分析（GSEA）也显示，在KIF2C高表达的LUAD样品中，“细胞周期信号通路”和“P53相关通路”显著富集（FDR < 0.05）。综上所述，通过共表达分析，发现KIF2C与LUAD的进展和预后相关，可能通过调节细胞周期信号通路导致预后不良。
Lung cancer is a malignant tumor with high morbidity and mortality, of which 80% is non-small cell lung cancer (NSCLC). And lung adenocarcinoma (LUAD) is the most important and common subtype in the NSCLC. In current study, the microarray data GSE31210 containing LUAD (n = 226) and normal lung tissue (n = 20) was analyzed to identify 965 differentially expressed genes, on which weighted gene co-expression network analysis was performed. Finally, it was confirmed that there was a significant correlation between brown module and LUAD stage. In the significant module, a total of 54 network hub genes were identified, and six of them were also identified as hub genes of the protein-protein interaction network. In validation, KIF2C showed a higher correlation with disease stage than other hub genes (p < 0.001, R2 = 0.955). Functional enrichment suggests that KIF2C is associated with cell mitosis and cell cycle. Combined with clinicopathological parameters, we found that the high expression of KIF2C is closely related to the relapse and tumor stage of LUAD. Survival analysis showed a significant reduction in overall survival in LUAD patients with high expression of KIF2C. Gene set enrichment analysis (GSEA) also showed that the "cell cycle signaling pathway" and "P53 related pathway" were significantly enriched in LUAD samples with high expression of KIF2C (FDR < 0.05). In conclusion, based on the co-expression analysis, KIF2C was identified in the association with progression and prognosis of LUAD, which might refer a poor prognosis probably by regulating cell cycle signaling pathway.