Fusion proteins comprising a Fusarium-specific antibody linked to antifungal peptides protect plants against a fungal pathogen
Fusion proteins comprising a Fusarium-specific antibody linked to antifungal peptides protect plants against a fungal pathogen
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DOI：
10.1038/nbt970
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发表时间：
2004-06-01
影响因子：
46.9
通讯作者：
Liao, YC
中科院分区：
文献类型：
--
作者：
Peschen, D;Li, HP;Liao, YC
在植物中表达识别病原菌特异性抗原的重组抗体已被认为是作物保护的一种策略。我们报道了含有镰刀菌特异的重组抗体的融合蛋白的表达，该抗体与三种抗真菌多肽(AFP)之一相连，作为一种保护植物免受真菌疾病侵染的方法。从一个具有免疫活性的噬菌体展示文库中分离到一种针对镰刀菌细胞表面展示抗原的鸡源性单链抗体。当与三种AFP中的任何一种融合时，这种重组抗体在体外抑制真菌生长。融合蛋白在转基因拟南芥植株中的表达对尖孢镰刀菌有较高的保护作用。而单独表达真菌特异性抗体或AFP的植物只表现出中等的抗性。我们的结果表明，抗体融合蛋白可以作为有效和通用的工具来保护作物免受真菌侵染。
In planta expression of recombinant antibodies recognizing pathogen-specific antigens has been proposed as a strategy for crop protection. We report the expression of fusion proteins comprising a Fusarium-specific recombinant antibody linked to one of three antifungal peptides (AFPs) as a method for protecting plants against fungal diseases. A chicken-derived single-chain antibody specific to antigens displayed on the Fusarium cell surface was isolated from a pooled immunocompetent phage display library. This recombinant antibody inhibited fungal growth in vitro when fused to any of the three AFPs. Expression of the fusion proteins in transgenic Arabidopsis thaliana plants conferred high levels of protection against Fusarium oxysporum f.sp. matthiolae, whereas plants expressing either the fungus-specific antibody or AFPs alone exhibited only moderate resistance. Our results demonstrate that antibody fusion proteins may be used as effective and versatile tools for the protection of crop plants against fungal infection.