Electron microscopic studies of induced cartilage development and calcification.
Electron microscopic studies of induced cartilage development and calcification.
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DOI：
10.1083/jcb.35.1.81
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发表时间：
1967-10
期刊：
影响因子：
--
通讯作者：
Anderson HC
中科院分区：
文献类型：
--
作者：
Anderson HC
将培养的人羊膜细胞（FL株）注射到可的松条件小鼠的大腿肌肉中，增殖形成离散的集落，在5天的时间内，这些集落被许多成纤维细胞覆盖。2-4天后，成纤维细胞带内出现软骨细胞和基质。在FL细胞注射后12天内，软骨基质钙化。软骨细胞非常相似的成纤维细胞，他们似乎是来自，并很容易区分FL细胞的突出ergastoplasm和高尔基复合体。软骨基质由随机排列的胶原原纤维（轴向周期为600 A，宽度为250 A）的独特毡制品组成，小的电子不透明的叶状基质颗粒从胶原原纤维延伸。基质钙化发生与类似羟基磷灰石的放射状排列的针状结构的沉积。密集的中心往往被确定在这些集群。对严重钙化区域的检查显示磷灰石样物质的融合团块。一般来说，诱导软骨形成和钙化的精细结构类似于先前在正常骨骺中描述的软骨发育和钙化。
Cultured, human, amniotic cells (FL strain) injected into the thigh muscles of cortisone-conditioned mice proliferated to form discrete colonies which, over a period of 5 days, became invested by numerous fibroblasts. Cartilage cells and matrix appeared within the fibroblastic zones during the succeeding 2–4 days. Cartilage matrix calcified within 12 days following FL-cell injection. Cartilage cells closely resembled fibroblasts from which they appeared to be derived, and were readily distinguished from FL cells by their prominent ergastoplasm and Golgi complexes. Cartilage matrix was composed of a distinctive feltwork of randomly arranged, collagen fibrils (∼600 A axial period and ∼250 A width) from which small electron-opaque, leaflike matrix particles extended. Matrix calcification occurred with the deposition of radially arranged needle-like structures resembling hydroxyapatite. Dense centers were often identified within these clusters. Examination of heavily calcified areas revealed confluent masses of apatite-like material. In general, the fine structure of induced cartilage formation and calcification resembled that of cartilage development and calcification as previously described in the normal epiphysis.