Isolation and characterization of the murine zinc finger coding gene, ZT2:: expression in normal and transformed myogenic cells
Isolation and characterization of the murine zinc finger coding gene, ZT2:: expression in normal and transformed myogenic cells
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DOI：
10.1016/s0378-1119(99)00044-x
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发表时间：
1999-04-01
期刊：
影响因子：
3.5
通讯作者：
Bouché, M
中科院分区：
文献类型：
--
作者：
Giorgi, S;Polimeni, M;Bouché, M
在一个旨在鉴定参与骨骼肌组织发生和分化的锌指蛋白的项目中，我们分离了一个小鼠基因，命名为ZT 2。2.44 kb的部分cDNA克隆对应于该基因的3'区，并含有0.54 kb的开放阅读框架，编码四个串联的C2H2样锌指结构域。该cDNA与多个转录物（2，4.5和7kb）杂交，其表达水平在不同组织中以及在同一组织的不同发育阶段中变化。至少在骨骼肌中，我们观察到不同发育阶段的转录本的聚腺苷酸化状态的差异。序列分析和遗传作图表明，ZT2与位于人Chr 11q23上的锌指基因ZNF 125同源。在人类中，在该染色体区域发现了高频率的肿瘤相关易位。正如预期的那样，ZT2映射到小鼠9号染色体上的相应区域。(C)1999 Elsevier Science B.V.保留所有权利。
In the context of a project aimed at the identification of zinc finger proteins involved in skeletal muscle histogenesis and differentiation, we isolated a murine gene, named ZT2. The 2.44 kb partial cDNA clone corresponds to the 3' region of the gene, and contains a 0.54 kb open reading frame encoding four C2H2-like zinc finger domains, organized in tandem. This cDNA hybridizes with multiple transcripts (2, 4.5 and 7 kb), whose expression levels vary in different tissues and at different developmental stages in the same tissue. At least in skeletal muscle we observed differences in the polyadenylation state of the transcripts at different stages of development. Moreover, ZT2 expression is correlated with cell proliferation and transformation.Sequence analysis and genetic mapping indicate that ZT2 is the homologue of ZNF125, one of the linked zinc finger encoding genes localized on human Chr 11q23. In humans, a high frequency of tumor-associated translocations is found in this chromosome region. As expected, ZT2 maps to the corresponding region on chromosome 9 in the mouse. (C) 1999 Elsevier Science B.V. All rights reserved.