EFFECTS OF EXERCISE ON INSULIN BINDING AND GLUCOSE-METABOLISM IN MUSCLE
EFFECTS OF EXERCISE ON INSULIN BINDING AND GLUCOSE-METABOLISM IN MUSCLE
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DOI：
10.1139/y84-248
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发表时间：
1984-01-01
影响因子：
2.1
通讯作者：
WATSONWRIGHT, WM
中科院分区：
文献类型：
--
作者：
BONEN, A;TAN, MH;WATSONWRIGHT, WM
为了阐明运动后肌肉增强胰岛素敏感性的机制，对小鼠跑步机运动 60 分钟后的比目鱼肌和趾长伸肌 (EDL) 肌肉中的胰岛素结合、2-脱氧-D-[1-14C]葡萄糖 (2-DOG) 摄取以及糖酵解和糖原生成中的 [5-3H] 葡萄糖利用进行了研究。在比目鱼肌中，运动后糖生成增加（P < 0.05），并且对胰岛素的作用保持敏感。运动后胰岛素刺激的比目鱼肌糖酵解也增加（P < 0.05）。在 EDL 中，运动后糖原生成增加（P < 0.05）。在没有胰岛素的情况下这已经是最大的并且没有被胰岛素进一步刺激（0.1-4 nM）。运动后（18°C 孵育 4 小时），比目鱼肌中葡萄糖摄取的处理没有改变。 37°C 孵育 1 小时。 C，在不存在胰岛素 (0 nM) 和存在胰岛素 (0.2-4 nM) 的情况下 (P < 0.05)，在比目鱼肌中观察到运动后 2-DOG 摄取显着增加 (P < 0.05)。 EDL 中也出现了类似的运动后 2-DOG 摄取增加。尽管葡萄糖摄取和代谢显着增加，但在 37°C 时，EDL 或比目鱼肌中的胰岛素结合没有明显变化。 C 或 18 度。 C. 运动后葡萄糖消耗的增加似乎不能直接归因于与慢肌和快肌结合的胰岛素的增加。运动后，两种类型肌肉的葡萄糖代谢增量明显不同。
To elucidate the mechanism of enhanced insulin sensitivity by muscle after exercise, insulin binding, 2-deoxy-D-[1-14C]glucose (2-DOG) uptake and [5-3H]glucose utilization in glycolysis and glycogenesis was studied in soleus and extensor digitorum longus (EDL) muscles of mice after 60 min of treadmill exercise. In the soleus, glycogenesis was increased after exercise (P < 0.05) and remained sensitive to the action of insulin. Postexercise insulin-stimulated glycolysis was also increased in the soleus (P < 0.05). In the EDL, glycogenesis was increased after exercise (P < 0.05). This was already maximal in the absence of insulin and was not further stimulated by insulin (0.1-4 nM). The disposal of glucose uptake was not altered in the soleus after exercise (4 h incubation at 18.degree. C). With 1-h incubations at 37.degree. C, a marked increase in 2-DOG uptake after exercise was observed in the soleus (P < 0.05) in the absence (0 nM) and presence of insulin (0.2-4 nM) (P < 0.05). A similar postexercise increase in 2-DOG uptake occurred in EDL. Despite the marked increase in glucose uptake and metabolism, no changes in insulin binding were apparent in either EDL or soleus at 37.degree. C or 18.degree. C. The postexercise increase of glucose disposal does not appear to be directly attributable to increments in insulin binding to slow-twitch and fast-twitch muscles. After exercise the increments in glucose metabolism differ markedly in the 2 types of muscle.