Further characterization of an aluminum influx transporter in rice
Further characterization of an aluminum influx transporter in rice
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DOI：
10.4161/psb.6.1.14319
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发表时间：
2011-01
影响因子：
2.9
通讯作者：
Jixing Xia;N. Yamaji;J. Ma
中科院分区：
文献类型：
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作者：
Jixing Xia;N. Yamaji;J. Ma
Nrat1是最近在水稻中发现的质膜定位铝转运蛋白，是Nramp家族的成员。在这里，我们进一步从转运底物特异性方面对这种转运蛋白进行了表征。酵母中Nrat1的转运活性不受高浓度Ca的影响，但被Al类似物Yb和Ga等三价离子显著抑制。敲除Nrat1不影响水稻对Cd和Mn的吸收。另一方面，与野生型水稻相比，Nrat1的过表达导致水稻根系对铝的吸收增强，但对镉的吸收没有影响。这些结果进一步证明，与其他Nramp成员不同，Nrat1是三价铝离子的内流转运体。
Nrat1 is a plasma membrane-localized aluminum transporter recently identified in rice, which is a member of Nramp family. Here, we further characterized this transporter in terms of transport substrate specificity. Heterologous assay in yeast showed that Al transport activity by Nrat1 was unaffected by the presence of high concentration of Ca, but significantly inhibited by trivalent ions including Yb and Ga, analogs of Al. Knockout of Nrat1 did not affect the uptake of Cd and Mn in rice. On the other hand, over-expression of Nrat1 led to enhanced Al uptake by rice roots compared with wild-type rice, but did not affect Cd uptake. These results provide further evidence that unlike other Nramp members, Nrat1 is an influx transporter for trivalent Al ion.