Intestinal Absorption and Tissue Distribution of Aza-Sugars from Mulberry Leaves and Evaluation of Their Transport by Sugar Transporters
Intestinal Absorption and Tissue Distribution of Aza-Sugars from Mulberry Leaves and Evaluation of Their Transport by Sugar Transporters
复制标题
桑叶氮杂糖的肠道吸收和组织分布以及糖转运蛋白转运的评价
DOI：
10.1021/acs.jafc.0c03005
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发表时间：
2020
影响因子：
6.1
通讯作者：
Nakagawa Kiyotaka
中科院分区：
文献类型：
--
作者：
Takasu Soo;Parida Isabella S;Ito Junya;Kojima Yoshihiro;Eitsuka Takahiro;Kimura Toshiyuki;Nakagawa Kiyotaka
桑葚富含氮杂糖，特别是1-脱氧野尻霉素（DNJ）、荞麦碱和2-O-α-d-吡喃半乳糖基-1-脱氧野尻霉素（GAL-DNJ），它们具有抗糖尿病和抗肥胖的特性。为了帮助我们了解氮杂糖的作用机制，药代动力学研究是必要的。因此，在本研究中，我们评估和比较了这些氮杂糖在大鼠中的吸收和器官分布。口服后，DNJ显示出最高的血浆浓度，其次是荞麦碱和GAL-DNJ。同时，DNJ和荞麦碱在器官中含量相似，而GAL-DNJ几乎未检测到，表明这些氮杂糖的吸收和分布特征存在差异。然后，我们研究了钠-葡萄糖协同转运蛋白和葡萄糖转运蛋白（GLUT）在氮杂糖转运中的作用，发现两者都参与DNJ转运，而荞麦碱的转运仅由GLUT促进。这些发现提供了深入了解这些氮杂糖的生物利用度和生物活性机制。
Mulberry leaves are rich in aza-sugars, particularly 1-deoxynojirimycin (DNJ), fagomine, and 2-O-α-d-galactopyranosyl-1-deoxynojirimycin (GAL-DNJ), which have antidiabetes and antiobesity properties. To help us understand the mechanisms of action of aza-sugars, pharmacokinetic studies are necessary. Therefore, in this study, we evaluated and compared the absorption and organ distribution of these aza-sugars in rats. Following oral intake, DNJ exhibited the highest plasma concentration followed by fagomine and GAL-DNJ. Meanwhile, similar amounts of DNJ and fagomine were present in organs, while GAL-DNJ was hardly detected, suggesting the diversity in absorption and distribution characteristics of these aza-sugars. We then investigated the role of the sodium-glucose cotransporter and the glucose transporter (GLUT) in the transport of aza-sugars and found that both are involved in DNJ transport, while transport of fagomine is solely facilitated by the GLUT. These findings provide insight into the bioavailability and bioactive mechanisms of these aza-sugars.