The isolation of cell nuclei in non-aqueous media.
The isolation of cell nuclei in non-aqueous media.
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DOI：
10.1085/jgp.35.3.529
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发表时间：
1952-01
期刊：
影响因子：
--
通讯作者：
SAETREN H
中科院分区：
文献类型：
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作者：
ALLFREY V;STERN H;MIRSKY AE;SAETREN H
1.本文介绍了一种改良的Behrens方法，用于从禽类红细胞和小牛或马的肝、肾、胸腺、胰腺、心脏和肠粘膜中分离细胞核。2.这些细胞核的纯度已通过染色反应、酶研究和血清蛋白免疫学试验确定。3.有证据表明，运输细胞质蛋白进入细胞核不会发生在隔离。4.在非水介质中制备的细胞核含有相当多的蛋白质和非常不同的酶组成，从在稀柠檬酸中通过“均质化”技术制备的细胞核中观察到的。5.讨论了在有机介质中制备的细胞核用于研究细胞内酶分布的适宜性。
1. A modified Behrens procedure is described for the isolation of nuclei from avian erythrocytes and from the liver, kidney, thymus, pancreas, heart, and intestinal mucosa of the calf or horse. 2. The purity of these nuclei has been established by staining reactions, enzyme studies, and immunological tests for serum proteins. 3. Evidence is presented to show that a transport of cytoplasmic proteins into the nucleus does not occur during the isolation. 4. Nuclei prepared in non-aqueous media contain considerably more protein and a very different enzyme composition from that observed in nuclei prepared by "homogenization" techniques in dilute citric acid. 5. The suitability of nuclei prepared in organic media for the study of intracellular enzyme distribution is discussed.