FLUORESCENCE POLARIZATION IMMUNOASSAY FOR THE DETERMINATION OF THERAPEUTIC DRUG LEVELS IN HUMAN-PLASMA
FLUORESCENCE POLARIZATION IMMUNOASSAY FOR THE DETERMINATION OF THERAPEUTIC DRUG LEVELS IN HUMAN-PLASMA
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DOI：
10.1093/jat/5.5.236
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发表时间：
1981-01-01
影响因子：
2.5
通讯作者：
JOLLEY, ME
中科院分区：
文献类型：
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作者：
JOLLEY, ME
荧光偏振免疫测定（FPIA）是一种已知多年的技术，但是尽管其具有许多优点，但尚未看到临床实用性。其主要原因是缺乏适用于临床实验室的简单、快速的高性能仪器。这一问题得到了解决，并制定了满足这些需要的文书。与该技术相关的另外两个问题的解决方案（即，示踪剂与血清蛋白的非特异性结合和血清的固有荧光）。开发了多种治疗药物（包括庆大霉素、茶碱、苯妥英和苯巴比妥）的测定方法，并显示与许多参考方法具有良好的相关性。该分析是完全自动化的，同时增加了速度和易用性，并在手动系统的性能显着改善。
Fluorescence polarization immunoassay (FPIA) is a technique which has been known for a number of years, but despite its many advantages, it has not seen clinical utility. The main reason for this is the lack of simple, rapid high performance instrumentation suitable for use in a clinical laboratory. This problem was addressed and an instrument developed which meets these needs. The solutions of the 2 other problems associated with the technique (i.e., non-specific binding of tracer to serum proteins and the intrinsic fluorescence of serum) were also accomplished. Assays for a variety of therapeutic drugs including gentamicin, theophylline, phenytoin and phenobarbital were developed and shown to correlate well with a number of reference methods. The assays were totally automated with a concomitant increase in speed and ease of use and a significant improvement in performance over the manual system.