Mapping T cell epitopes by flow cytometry
Mapping T cell epitopes by flow cytometry
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DOI：
10.1016/s1046-2023(02)00349-3
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发表时间：
2003-03-01
期刊：
影响因子：
4.8
通讯作者：
Kern, F
中科院分区：
文献类型：
--
作者：
Hoffmeister, B;Kiecker, F;Kern, F
通过流式细胞术绘制表位图是一种非常现代的方法，它不仅识别T细胞表位，同时还允许详细分析响应的T细胞亚群，包括谱系、激活标记表达和其他感兴趣的标记。最常用的方法是在多肽或多肽库刺激后，识别分泌抑制激活的T细胞中的细胞内细胞因子。最近开发的一种方法通过测量增殖细胞中羧基荧光素二乙酸酯琥珀酰亚胺酯染色的减少来分析T细胞的增殖。这篇文章包括关于多肽构型的信息，关于多肽库的设计和有效应用的一节，以及这两种分析的实验室工作方案。(C)2003年埃尔塞维尔科学公司(美国)。版权所有。
Epitope mapping by flow cytometry is a very modern approach that not only identifies T-cell epitopes but simultaneously allows for detailed analysis of the responding T-cell subsets including lineage, activation marker expression, and other markers of interest. The most frequently used approach is based on the identification of intracellular cytokines in secretion-inhibited activated T cells following stimulation with peptides or peptide pools. A more recently developed assay analyzes T-cell proliferation by measuring the decrease in carboxyfluorescein diacetate succinimidyl ester staining in proliferated cells. This article includes information on peptide configuration, a section on the design and efficient application of peptide pools, and working laboratory protocols for both assays. (C) 2003 Elsevier Science (USA). All rights reserved.