Optimization of electrospun TSF nanofiber alignment and diameter to promote growth and migration of mesenchymal stem cells
Optimization of electrospun TSF nanofiber alignment and diameter to promote growth and migration of mesenchymal stem cells
复制标题
优化电纺 TSF 纳米纤维排列和直径以促进间充质干细胞的生长和迁移
DOI：
10.1016/j.apsusc.2012.08.008
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发表时间：
2012-11-15
影响因子：
6.7
通讯作者：
Zhang, Huanxiang
中科院分区：
文献类型：
--
作者：
Qu, Jing;Zhou, Dandan;Zhang, Huanxiang
丝素蛋白支架是一种天然来源的生物相容性基质，具有重建外科应用的潜力。本研究采用静电纺丝法制备了不同直径（400 nm、800 nm和1200 nm）和排列（随机和排列）的柞蚕丝素蛋白（TSF）支架，并进一步研究了间充质干细胞（MSCs）在这些支架上的生长和迁移。CD90免疫荧光染色显示纤维排列对MSCs的形态有很大的影响，表明支架的排列可以决定细胞的分布。与800 nm和1200 nm的无规TSF支架相比，直径较小、排列整齐的TSF支架更有利于MSCs的生长。此外，三维TSF诱导的MSCs迁移速度和效率增加，突出了TSF对迁移的MSCs的指导作用。更重要的是，与较大直径的TSF支架相比，400 nm对齐的TSF支架显著提高了细胞迁移速度，并进一步诱导了MSC的最有效迁移。总之，数据表明，较小的直径和对齐的静电纺丝TSF代表了支持和促进MSC生长和迁移的有价值的支架，从而提高了操纵TSF支架以增强MSC在细胞治疗中的归巢和治疗潜力的可能性。(C)2012年爱思唯尔B。V.保留所有权利。
Silk fibroin scaffolds are a naturally derived biocompatible matrix with the potential for reconstructive surgical applications. In this study, tussah silk fibroin (TSF) nanofiber with different diameters (400 nm, 800 nm and 1200 nm) and alignment (random and aligned) were prepared by electrospinning, then the growth and migration of mesenchymal stem cells (MSCs) on these materials were further evaluated. CD90 immunofluorescence staining showed that fiber alignment exhibited a strong influence on the morphology of MSCs, indicating that the alignment of the scaffolds could determine the distribution of cells. Moreover, smaller diameter and aligned TSF scaffolds are more favorable to the growth of MSCs as compared with 800 nm and 1200 nm random TSF scaffolds. In addition, the increased migration speed and efficiency of MSCs induced by three-D TSF were verified, highlighting the guiding roles of TSF to the migrated MSCs. More importantly, 400 nm aligned TSF scaffolds dramatically improved cell migratory speed and further induced the most efficient migration of MSCs as compared with larger diameter TSF scaffolds. In conclusion, the data demonstrate that smaller diameter and aligned electrospun TSF represent valuable scaffolds for supporting and promoting MSCs growth and migration, thus raising the possibility of manipulating TSF scaffolds to enhance homing and therapeutic potential of MSCs in cellular therapy. (C) 2012 Elsevier B. V. All rights reserved.