Splicing and editing of rps10 transcripts in potato mitochondria
Splicing and editing of rps10 transcripts in potato mitochondria
复制标题
马铃薯线粒体中rps10转录本的剪接和编辑
作者：
S. Zanlungo;V. Quiñones;A. Moenne;L. Holuigue;X. Jordana
编码核糖体蛋白 S10 的马铃薯线粒体基因 rps10 的结构和表达已得到表征。 RPS10 多肽由 129 个氨基酸组成，分别由 307 bp 和 80 bp 的两个外显子编码，两个外显子由 774 bp 的 II 类内含子分隔。通过剪接 cDNA 的序列分析研究了完整 rps10 编码区的编辑。四个 C 残基被编辑成 U，从而产生了一个推定的翻译起始密码子、一个消除了十个羧基末端残基的新终止密码子以及两个额外的氨基酸改变。所有这些变化增加了马铃薯和地钱多肽之间的相似性。在未剪接的 cDNA 的内含子序列中观察到的一个额外的 C-to-U RNA 编辑事件提高了结构域 I 的茎 I (i) 二级结构的稳定性，因此可能是剪接反应所必需的。所有剪接的 cDNA 和大多数未剪接的 cDNA 均被完全编辑，这表明编辑是 rps10 mRNA 加工的早期步骤，并且发生在剪接之前。目前已知，马铃薯 rps10 的早期工作（Zanlungo 等人，1994）仅包含该基因的部分分析，因为没有识别出短的下游外显子。
The structure and expression of the potato mitochondrial gene rps10, encoding ribosomal protein S10, has been characterized. The RPS10 polypeptide of 129 amino acids is encoded by two exons of 307 bp and 80 bp respectively, which are separated by a 774-bp class-II intron. Editing of the complete rps10 coding region was studied by sequence analysis of spliced cDNAs. Four C residues are edited into U, resulting in the creation of a putative translational initiation codon, a new stop codon which eliminated ten carboxy-terminal residues, and two additional amino-acid alterations. All these changes increase the similarity between the potato and liverwort polypeptides. One additional C-to-U RNA editing event, observed in the intron sequence of unspliced cDNAs, improves the stability of the secondary structure in stem I (i) of domain I and may thus be required for the splicing reaction. All spliced cDNAs, and most unspliced cDNAs, were completely edited, suggesting that editing is an early step of rps10 mRNA processing and precedes splicing. Earlier work on potato rps10 (Zanlungo et al. 1994) is now known to comprise only a partial analysis of the gene, since the short downstream exon was not identified.
影响因子：
11.4
作者：
HUNT, MD;NEWTON, KJ
通讯作者：
NEWTON, KJ