Differential neurogenesis and gliogenesis by local and migrating neural stem cells in the olfactory bulb
Differential neurogenesis and gliogenesis by local and migrating neural stem cells in the olfactory bulb
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DOI：
10.1016/s0168-0102(02)00173-6
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发表时间：
2002-12-01
影响因子：
2.9
通讯作者：
Moriizumi, T
中科院分区：
文献类型：
--
作者：
Fukushima, N;Yokouchi, K;Moriizumi, T
吻侧迁移流（RMS）是一种独特的前脑结构，为脑室周围区域的神经干细胞向嗅球（013）提供长距离迁移路线。本研究的目的是检查 OB 中不同来源（脑室周围与球内）的神经干细胞的神经发生和胶质细胞生成的程度。 RMS损伤后，大鼠腹腔注射5-溴脱氧尿苷(BrdU)，并继续饲养2周。使用针对损伤和未损伤（对照）RMS 的 OB 中的神经元（NeuN，神经元核）和神经胶质（GFAP，神经胶质纤维酸性蛋白）标记物的抗体，通过免疫组织化学方法检查嗅球颗粒细胞（OB-GCL）和嗅肾小球（013-GL）层中 BrdU（+）细胞的神经元和神经胶质分化。在完全RMS损伤的013中，脑室周围神经干细胞的迁移受到抑制，在OB-GCL和OB-GL中均发现少量BrdU(+) NeuN(+)细胞。对照侧（OB-GCL，36.7%；OB-GL，8.8%）的 BrdU（+）NeuN（+）细胞占 BrdU（+）细胞的比例比完全 RNIS 损伤侧（OB-GCL，3.7%；OB-GL，0.6%）高得多。 BrdU(+) GFAP(+) 细胞相对于 BrdU(+) 细胞的百分比在对照侧和完全 RMS 损伤侧之间没有显示出任何重大差异。这项研究揭示了成年啮齿动物 OB 中局部神经干细胞和迁移神经干细胞之间神经发生和胶质细胞发生的差异。 (C) 2002 Elsevier Science Ireland Ltd. 和日本神经科学学会。版权所有。
The rostral migratory stream (RMS) is a unique forebrain structure that provides a long-distance migratory route for the neural stem cells of the periventricular region towards the olfactory bulb (013). The purpose of the study presented here is to examine the extent of neurogenesis and gliogenesis by the neural stem cells of different origins (periventricular vs. intrabulbar) in the OB. After the RMS had been subjected to injury, the rats received intraperitoneal injections of 5-bromodeoxyuridine (BrdU) and were further reared for 2 weeks. Neuronal and glial differentiations of the BrdU(+) cells in the olfactory bulbar granule cell (OB-GCL) and the olfactory glomerular (013-GL) layers were examined immunohistochemically using antibodies against neuronal (NeuN, neuronal nuclei) and glial (GFAP, glial fibrillary acidic protein) markers in the OBs with injured and uninjured (control) RMS. In the completely RMS-lesioned 013, where migration of the periventricular neural stem cells was inhibited, a small number of BrdU(+) NeuN(+) cells were found in both the OB-GCL and OB-GL. The BrdU(+) NeuN(+) cells accounted for a much higher percentage of the BrdU(+) cells on the control side (OB-GCL, 36.7%; OB-GL, 8.8%) than on the completely RNIS-lesioned side (OB-GCL, 3.7%; OB-GL, 0.6%). The percentage of the BrdU(+) GFAP(+) cells relative to the BrdU(+) cells did not show any major difference between the control and completely RMS-lesioned sides. This study revealed differences in neurogenesis and gliogenesis between the local and migrating neural stem cells in the OB of the adult rodent. (C) 2002 Elsevier Science Ireland Ltd. and the Japan Neuroscience Society. All rights reserved.