SSR-based genetic maps of Miscanthus sinensis and M. sacchariflorus, and their comparison to sorghum
SSR-based genetic maps of Miscanthus sinensis and M. sacchariflorus, and their comparison to sorghum
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DOI：
10.1007/s00122-012-1790-1
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发表时间：
2012-05-01
影响因子：
5.4
通讯作者：
Paterson, Andrew H.
中科院分区：
文献类型：
--
作者：
Kim, Changsoo;Zhang, Dong;Paterson, Andrew H.
我们展示了一种基于ssr的遗传图谱，该图谱来自Miscanthus sacchariflorus Robustus和M. sinensis的杂交，后者是有前途的纤维素生物燃料原料Miscanthus x giganteus的祖先。由于各亲本种的高杂合性，SSR标记采用双向伪检验交模型进行定位。在M. sacchariflorus中共定位了261个位点，跨越40个连锁群，全长1998.8 cM，覆盖了72.7%的基因组。中华支原体共定位到303个位点，形成23个连锁群，全长2238.3 cM，覆盖84.9%的基因组。利用dna衍生的SSR位点，可以将芒草连锁群与高粱染色体进行比对，揭示了在高粱亚族和糖精亚族分化后影响芒草谱系的全基因组重复，以及泛谷物全基因组重复的痕迹。虽然目前的图谱提供了对这种新兴作物的许多早期研究需求，但还需要额外的标记来提高图谱密度，并进一步表征芒草基因组自与高粱和甘蔗分离以来的结构变化。
We present SSR-based genetic maps from a cross between Miscanthus sacchariflorus Robustus and M. sinensis, the progenitors of the promising cellulosic biofuel feedstock Miscanthus x giganteus. cDNA-derived SSR markers were mapped by the two-way pseudo-testcross model due to the high heterozygosity of each parental species. A total of 261 loci were mapped in M. sacchariflorus, spanning 40 linkage groups and 1,998.8 cM, covering an estimated 72.7% of the genome. For M. sinensis, a total of 303 loci were mapped, forming 23 linkage groups and 2,238.3 cM, covering 84.9% of the genome. The use of cDNA-derived SSR loci permitted alignment of the Miscanthus linkage groups to the sorghum chromosomes, revealing a whole genome duplication affecting the Miscanthus lineage after the divergence of subtribes Sorghinae and Saccharinae, as well as traces of the pan-cereal whole genome duplication. While the present maps provide for many early research needs in this emerging crop, additional markers are also needed to improve map density and to further characterize the structural changes of the Miscanthus genome since its divergence from sorghum and Saccharum.