Dynamic molecular combing: Stretching the whole human genome for high-resolution studies
Dynamic molecular combing: Stretching the whole human genome for high-resolution studies
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DOI：
10.1126/science.277.5331.1518
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发表时间：
1997-09-05
期刊：
影响因子：
56.9
通讯作者：
Bensimon, A
中科院分区：
文献类型：
--
作者：
Michalet, X;Ekong, R;Bensimon, A
代表数百个真核生物基因组的DNA量通过动态分子梳理在硅烷化表面上延伸。杂交的DNA探针的精确测量直接实现，而不需要归一化。该方法通过酵母人工染色体（YAC）上粘粒重叠群在酵母基因组DNA内的高分辨率定位得到验证。它被扩展到人类基因组DNA的精确测量范围从T到150个碱基，重叠群内的缺口，以及患者DNA上结节性硬化症2基因的微缺失。这种方法的简单性，可重复性和精确性使其成为各种基因组研究的有力工具。
DNA in amounts representative of hundreds of eukaryotic genomes was extended on silanized surfaces by dynamic molecular combing. The precise measurement of hybridized DNA probes was achieved directly without requiring normalization. This approach was validated with the high-resolution mapping of cosmid contigs on a yeast artificial chromosome (YAC) within yeast genomic DNA. It was extended to human genomic DNA for precise measurements ranging from T to 150 kilobases, of gaps within a contig, and of microdeletions in the tuberous sclerosis 2 gene on patients' DNA. The simplicity, reproducibility, and precision of this approach makes it a powerful tool for a variety of genomic studies.