OsCDPKI3, a calcium-dependent protein kinase gene from rice, is induced by cold and gibberellin in rice leaf sheath
OsCDPKI3, a calcium-dependent protein kinase gene from rice, is induced by cold and gibberellin in rice leaf sheath
复制标题
DOI：
10.1007/s11103-004-1178-y
复制
发表时间：
2004-07-01
影响因子：
5.1
通讯作者：
Komatsu, S
中科院分区：
文献类型：
--
作者：
Abbasi, F;Onodera, H;Komatsu, S
钙依赖性蛋白激酶（CDPK）在水稻信号转导中起着重要作用，但每种CDPK的确切作用仍然很大程度上未知。最近，从水稻幼苗中分离到了一个编码OsCDPK13的全长cDNA。为了表征OsCDPK13的功能，本研究分析了其对各种应激和激素的反应。OsCDPK13在2周龄叶鞘和愈伤组织中积累，并在寒冷和赤霉素（GA）的作用下发生磷酸化。在GA(3)处理下，OsCDPK13基因表达和蛋白积累上调，但在脱落酸和油菜素内酯处理下受到抑制。反义OsCDPK13转基因水稻品系比载体对照品系短，且OsCDPK13在水稻矮化突变体中的表达量低于野生型。此外，OsCDPK13基因表达和蛋白积累在低温胁迫下增强，但在盐和干旱胁迫下受到抑制。Sense oscdpk13转基因水稻在低温胁迫下的恢复率高于病媒介对照水稻。OsCDPK13在耐寒水稻品种中的表达强于冷敏水稻品种。结果表明，OsCDPK13可能是水稻对赤霉素和冷胁迫响应的重要信号成分。
Calcium-dependent protein kinases (CDPKs) play an important role in rice signal transduction, but the precise role of each individual CDPK is still largely unknown. Recently, a full-length cDNA encoding OsCDPK13 from rice seedling was isolated. To characterize the function of OsCDPK13, its responses to various stresses and hormones were analyzed in this study. OsCDPK13 accumulated in 2-week-old leaf sheath and callus, and became phosphorylated in response to cold and gibberellin (GA). OsCDPK13 gene expression and protein accumulation were up-regulated in response to GA(3) treatment, but suppressed in response to abscisic acid and brassinolide. Antisense OsCDPK13 transgenic rice lines were shorter than the vector control lines, and the expression of OsCDPK 13 was lower in dwarf mutants of rice than in wild type. Furthermore, OsCDPK13 gene expression and protein accumulation were enhanced in response to cold, but suppressed under salt and drought stresses. Sense OsCDPK 13 transgenic rice lines had higher recovery rates after cold stress than vector control rice. The expression of OsCDPK13 was stronger in cold-tolerant rice varieties than in cold-sensitive ones. The results suggest that OsCDPK13 might be an important signaling component in the response of rice to GA and cold stress.