Co-occurrence of nitrite-dependent anaerobic methane oxidizing and anaerobic ammonia oxidizing bacteria in two Qinghai-Tibetan saline lakes
Co-occurrence of nitrite-dependent anaerobic methane oxidizing and anaerobic ammonia oxidizing bacteria in two Qinghai-Tibetan saline lakes
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DOI：
10.1007/s11707-012-0336-9
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发表时间：
2012-12
影响因子：
2
通讯作者：
Jian Yang;Hongchen Jiang;Geng Wu;Weiguo Hou;Yongjuan Sun;Z. Lai;Hailiang Dong
中科院分区：
文献类型：
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作者：
Jian Yang;Hongchen Jiang;Geng Wu;Weiguo Hou;Yongjuan Sun;Z. Lai;Hailiang Dong
依赖亚硝酸盐的厌氧甲烷氧化（n-damo）细菌和厌氧氨氧化（anammox）细菌是参与全球碳氮循环的两类微生物。从两个样品中成功提取了n-damo和厌氧氨氧化菌的DNA和cdna克隆，表明这两类细菌可以在盐度高达84 g/L的天然盐水环境中共存。我们的发现对我们理解自然界中全球碳和氮循环具有重大意义。
Nitrite-dependent anaerobic methane-oxidizing (n-damo) bacteria and anaerobic ammonia oxidizing (anammox) bacteria are two groups of microorganisms involved in global carbon and nitrogen cycling. In order to test whether the n-damo and anammox bacteria co-occur in natural saline environments, the DNA and cDNA samples obtained from the surficial sediments of two saline lakes (with salinity of 32 and 84 g/L, respectively) on the Tibetan Plateau were PCR-amplified with the use of anammox- and n-damo-specific primer sets, followed by clone library construction and phylogenetic analysis. DNA and cDNA-based clones affiliated with n-damo and anammox bacteria were successfully retrieved from the two samples, indicating that these two groups of bacteria can co-occur in natural saline environments with salinity as high as 84 g/L. Our finding has great implications for our understanding of the global carbon and nitrogen cycle in nature.