Studying histone inheritance in different systems using imaging-based methods and perspectives.
Studying histone inheritance in different systems using imaging-based methods and perspectives.
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DOI：
10.1042/bst20220983
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发表时间：
2023-06-28
影响因子：
3.9
通讯作者：
中科院分区：
文献类型：
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作者：
了解细胞身份在细胞和发育生物学领域至关重要。在细胞分裂过程中，母细胞复制遗传物质和细胞成分，产生两个子细胞。虽然两种细胞接收相同的遗传信息，但它们可以采取相似或不同的细胞命运，这是由对称或不对称分裂造成的。这些命运可以通过表观遗传机制来调节，这些机制可以改变基因表达而不改变遗传信息。组蛋白将DNA包裹成染色质的基本单位，是表观遗传信息的主要载体，并且可以通过与DNA的相互作用直接影响基因表达和其他细胞功能。虽然已经很好地研究了遗传信息是如何复制和分离的，但表观遗传信息（如组蛋白）如何通过细胞分裂遗传仍然是一个研究领域。由于典型的组蛋白在DNA复制过程中被掺入染色质中，并且可以随着时间的推移而被修饰，因此在细胞周期的背景下研究它们的遗传是很重要的。在这里，我们概述了组蛋白遗传的生物学基础以及可用于研究这一过程的基于成像的实验设计。此外，我们还讨论了各种研究，调查这种现象的重点是不对称分裂细胞在不同的系统。这一概要提供了深入了解组蛋白遗传的背景下，细胞周期，沿着的技术方法和考虑，必须采取时，在体内研究这一进程。
Understanding cell identity is critically important in the fields of cell and developmental biology. During cell division, a mother cell duplicates the genetic material and cellular components to give rise to two daughter cells. While both cells receive the same genetic information, they can take on similar or different cell fates, resulting from a symmetric or asymmetric division. These fates can be modulated by epigenetic mechanisms that can alter gene expression without changing genetic information. Histone proteins, which wrap DNA into fundamental units of chromatin, are major carriers of epigenetic information and can directly influence gene expression and other cellular functions through their interactions with DNA. While it has been well studied how the genetic information is duplicated and segregated, how epigenetic information, such as histones, are inherited through cell division is still an area of investigation. Since canonical histone proteins are incorporated into chromatin during DNA replication and can be modified over time, it is important to study their inheritance within the context of the cell cycle. Here, we outline the biological basis of histone inheritance as well as the imaging-based experimental design that can be used to study this process. Furthermore, we discuss various studies that have investigated this phenomenon with the focus on asymmetrically dividing cells in different systems. This synopsis provides insight into histone inheritance within the context of the cell cycle, along with the technical methods and considerations that must be taken when studying this process in vivo.
影响因子：
9.8
作者：
通讯作者：
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影响因子：
1.2
作者：
Huh, Yang Hoon;Noh, Minsoo;Burden, Frank R.;Chen, Jennifer C.;Winkler, David A.;Sherley, James L.
通讯作者：
Sherley, James L.