Retrovirus-mediated human heme oxygenase-1 (HO-1) gene transfer into rat endothelial cells:: the effect of HO-1 inducers on the expression of cytokines
Retrovirus-mediated human heme oxygenase-1 (HO-1) gene transfer into rat endothelial cells:: the effect of HO-1 inducers on the expression of cytokines
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DOI：
10.1016/s1357-2725(02)00172-3
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发表时间：
2003-03-01
影响因子：
4
通讯作者：
Kamal, N
中科院分区：
文献类型：
--
作者：
Abdel-Aziz, MT;El-Asmar, MF;Kamal, N
本研究旨在探讨人血红素氧合酶-1（HO-1）是否通过诱导血管内皮生长因子（VEGF）介导血管生成。此外，在存在和不存在HO-1诱导剂的情况下研究了HO-1对转化生长因子β（TGF-β）表达的影响。mRNA提取和逆转录聚合酶链反应（RT-PCR）实验，以评估HO-1，VEGF和TGF-β的表达在HO诱导剂包括H2 O2，内毒素和蛇毒金属蛋白酶与去整合素样活性（SnMP）的存在和不存在下。ELISA法检测转染HO-1的内皮细胞中VEGF的表达，结果显示转染HO-1的内皮细胞中VEGF的表达明显高于未转染HO-1的内皮细胞。另一方面，与对照非转导细胞相比，HO-1转导的内皮细胞中TGF-β的表达及其蛋白水平被显著抑制。内毒素和SnMP对HO-1转导的内皮细胞中VEGF的表达和TGF-β的抑制表现出更显著的作用，这表明它们的作用最可能是通过诱导HO-1介导的。(C)2002爱思唯尔科技有限公司版权所有。
The present study was conducted to investigate if the mechanism of human heme oxygenase-1(HO-1) mediated angiogenesis was through the induction of vascular endothelial growth factor (VEGF). Also, the effect of HO-1 on the expression of transforming growth factor beta (TGF-beta),was studied in the presence and absence of HO-1 inducers.Rat lung microvessel endothelial cell line transduced with human HO-1 gene was subjected to cell culture (six separate experiments). mRNA extraction and reverse transcriptase polymerase chain reaction (RT-PCR) experiments, were performed to evaluate the expression of HO-1, VEGF, and TGF-beta in the presence and absence of HO inducers including H2O2, endotoxin and snake venoum metalloproteinase with disintegrin like activity(SnMP). ELISA technique was performed to evaluate the levels of the studied growth factors.The results of the study showed over expression of VEGF in endothelial cells transduced with HO-1 compared to control non-transduced endothelial cells. On the other hand, the expression of TGF-beta and its protein level were markedly inhibited in HO-1 transduced endothelial cells compared to control non-transduced cells. Endotoxin and SnMP showed more prominent effect on the expression of VEGF and suppression of TGF-beta in HO-1 transduced endothelial cells, suggesting that their effect is most probably mediated through induction of HO-1. (C) 2002 Elsevier Science Ltd. All rights reserved.