Age Changes in Secretory Function of Male and Female Rat Parotid Glands in Response to Methoxamine and Pilocarpine
Age Changes in Secretory Function of Male and Female Rat Parotid Glands in Response to Methoxamine and Pilocarpine
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甲氧明和毛果芸香碱对雄性和雌性大鼠腮腺分泌功能的年龄变化的影响
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
K. Abe
中科院分区：
文献类型：
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作者：
A. Inanaga;T. Habu;E. Tanaka;T. Taniguch;T. Nishiura;K. Ishibashi;S. Naruse;K. Abe
唾液分泌的甲氧胺和毛果芸香碱的雄性和雌性大鼠腮腺的导管中收集的每周年龄间隔从3至10周，并在3.5，8和15个月的年龄。分析蛋白质、钾、钙、无机磷酸盐的浓度和淀粉酶活性。蛋白质的类型进行了测定，并进行了氨基酸分析的总蛋白质。腺体的湿重大幅度增加到8周龄，然后达到几乎平台值，最后在15个月龄时趋于下降，在两种性别。唾液分泌量的甲氧胺和毛果芸香碱与腮腺重量在两种性别呈正相关。蛋白质，钾和无机磷酸盐的浓度呈负相关的唾液流量只有在相对较低的流量。淀粉酶活性与蛋白质浓度呈正相关，与刺激物的性质、年龄和性别无关。以甲氧胺为刺激物，淀粉酶活性与钙浓度呈正相关，与年龄和性别无关。蛋白质和氨基酸浓度的类型是独立的性质的刺激，年龄和性别长达15个月的年龄。然而，在腮腺唾液中的几个大鼠在8个月和15个月的年龄，观察到不寻常的蛋白质，独立的性质的刺激和性别。
Saliva secreted in response to methoxamine and pilocarpine was collected from the cannulated ducts of both parotid glands of male and female rats at weekly age intervals from three to 10 weeks, and at 3.5, 8, and 15 months of age. It was analyzed for the concentrations of protein, potassium, calcium, inorganic phosphate, and for amylase activity. The types of protein were determined electrophoretically, and an amino acid analysis of the total protein was also carried out. The wet weights of the glands increased substantially up to eight weeks of age, then reached almost plateau values, and finally tended to decrease at 15 months of age in both sexes. The salivary volumes secreted in response to methoxamine and pilocarpine were positively correlated with the parotid gland weights in both sexes. The concentrations of protein, potassium, and inorganic phosphate were inversely related to the salivary flow rates only at relatively low rates of flow. The amylase activity was positively correlated with the concentration of protein, independent of the nature of the stimulus, age, and sex. With methoxamine as a stimulus, the amylase activity was positively correlated with the concentration of calcium, independent of age and sex. The types of protein and amino acid concentrations were independent of the nature of the stimulus, age, and sex up to 15 months of age. However, in parotid saliva of several rats at 8 and 15 months of age, unusual proteins were observed electrophoretically, independent of the nature of the stimulus and sex.