Perinatal transmission of HIV type 1: associations with maternal anti-HIV serological reactivity. Mothers and Infants Cohort Study and the HIV-1 Perinatal Serology Working Group.
Perinatal transmission of HIV type 1: associations with maternal anti-HIV serological reactivity. Mothers and Infants Cohort Study and the HIV-1 Perinatal Serology Working Group.
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1 型 HIV 的围产期传播：与母亲抗 HIV 血清学反应性的关联。
DOI：
10.1089/aid.1994.10.1125
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发表时间：
1994
影响因子：
1.5
通讯作者：
Dublin,S
中科院分区：
文献类型：
--
作者：
Goedert,JJ;Dublin,S
作为一项对人类免疫缺陷病毒1型（HIV-1）包膜大区域的假设生成研究，我们与几个实验室合作，检测了65名HIV-1阳性孕妇的血清，其中18名（28%）将病毒传播给婴儿。试验包括HIV LAI中和抗体和对102种HIV-1 Env肽的反应性，这些肽的序列基于毒株LAI、MN、SC、RF和WMJ-2以及几种临床分离株，涵盖约65%的gp 120和约80%的gp 41。V3环和中和活性的结果相互矛盾，大部分未达到统计学显著性。与几个gp 41表位（氨基酸571-585，736-750，也许650-663）反应的传播风险似乎较低，而与两个gp 120表位（氨基酸466-480和475-486）和一个gp 41表位（氨基酸547-576）反应的风险似乎较高。然而，这些关联可能只是偶然发生的，因为测试了如此大量的肽。对于独立合成的肽，实验室之间的结果往往不一致。然而，当使用相同的方案和肽时，再现性良好（秩相关系数≥0.78）。虽然这项研究不能确定一个体液免疫应答线性Env肽，一贯和广泛的保护围产期传播的HIV-1，有gp 120和gp 41的区域，应在更大的队列和技术进行评估，以调查潜在的构象表位和中和自体或临床分离的HIV-1的社区。
As a hypothesis-generating study of large regions of the human immunodeficiency virus type 1 (HIV-1) envelope, we collaborated with several laboratories to test sera from subgroups of 65 HIV-1-positive pregnant women, 18 (28%) of whom transmitted the virus to their infants. Assays included neutralizing antibodies to HIVLAIand reactivity to 102 HIV-1 Env peptides with sequences based on strains LAI, MN, SC, RF, and WMJ-2 as well as several clinical isolates, spanning about 65% of gp120 and about 80% of gp41. Results for the V3 loop and for neutralizing activity were conflicting and for the most part did not reach statistical significance. Transmission risk appeared lower with reactivity to a few gp41 epitopes (amino acids 571–585, 736–750, and perhaps 650–663), whereas risk appeared higher with reactivity to two gp120 epitopes (amino acids 466–480 and 475–486) and one gp41 epitope (amino acids 547–576). However, these associations could have occurred simply by chance because such a large number of peptides was tested. With independently synthesized peptides, results between laboratories often were inconsistent. However, reproducibility was good (rank correlation coefficient ≥0.78) when the same protocols and peptides were used. Although this study could not identify a humoral immune response to linear Env peptides that consistently and broadly protected against perinatal transmission of HIV-1, there were regions of gp120 and gp41 that should be evaluated in larger cohorts and with techniques to investigate potential conformational epitopes and neutralization to autologous or clinical isolates of HIV-1 from the community.