snail expression during embryonic development of the coral Acropora:: blurring the diploblast/triploblast divide?
snail expression during embryonic development of the coral Acropora:: blurring the diploblast/triploblast divide?
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DOI：
10.1007/s00427-004-0398-0
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发表时间：
2004-05-01
影响因子：
2.4
通讯作者：
Ball, EE
中科院分区：
文献类型：
--
作者：
Hayward, DC;Miller, DJ;Ball, EE
尽管珊瑚名义上是双胚细胞，但千叶鹿角珊瑚的早期发育过程明显类似于高等后生动物的原肠胚形成。这种形态水平上的相似性促使我们寻找鹿角珊瑚的等价基因，这些基因在原肠胚形成中的关键作用在高等后生动物中是保守的。我们在此报告了一种这样的基因（snail）的特征，该基因在果蝇和小鼠中都在经历上皮-间质转变和/或形态发生运动的细胞中表达。除了 N 端 SNAG 结构域外，卫城蜗牛蛋白还包含四个锌指，其序列可诊断蜗牛蛋白亚家族的成员。原位杂交揭示了扁平的原肠胚形成前胚胎一侧中央部分的上皮组织中的表达，当它被相对层吞没时，它继续表达 snail。与果蝇等两侧对称动物原肠胚形成过程中的蜗牛表达进行比较，揭示了惊人的相似性，并表明两侧对称动物的中胚层形成过程和刺胞动物中的内胚层形成过程之间存在机制上的、可能是进化上的联系。
Although corals are nominally diploblastic, the early development of Acropora millepora involves a process that clearly resembles gastrulation in higher metazoans. This similarity at the morphological level led us to search for the Acropora equivalents of genes whose key roles in gastrulation are conserved across the higher Metazoa. We here report the characterisation of one such gene, snail, which in both Drosophila and the mouse is expressed in cells undergoing an epithelial-mesenchyme transition and/or morphogenetic movements. In addition to an N-terminal SNAG domain, the Acropora snail protein contains four zinc fingers with sequences diagnostic for members of the snail protein subfamily. In situ hybridisation reveals expression in epithelial tissue in the central portion of one side of the flattened pre-gastrulation embryo, which continues to express snail as it is engulfed by its opposite layer. Comparison to snail expression during gastrulation in bilaterians such as Drosophila reveals striking similarities and suggests mechanistic, and possibly evolutionary, links between the processes of mesoderm formation in bilaterians and endoderm formation in the Cnidaria.