The role of ADAM-like decysin 1 in non-eosinophilic chronic rhinosinusitis with nasal polyps
The role of ADAM-like decysin 1 in non-eosinophilic chronic rhinosinusitis with nasal polyps
复制标题
DOI：
10.1080/00016489.2018.1481296
复制
发表时间：
2018-01-01
影响因子：
1.4
通讯作者：
Haruna, Shin-Ichi
中科院分区：
文献类型：
--
作者：
Sugimoto, Naoki;Nakayama, Tsuguhisa;Haruna, Shin-Ichi
背景：慢性鼻窦炎伴鼻息肉（CRSwNP）分为两种亚型：嗜酸性粒细胞性（ECRSwNP）和非嗜酸性粒细胞性（NECRSwNP）。尽管 ECRSwNP 的炎症模式已被阐明，但对 NECRSwNP 的了解却很少。目的：据报道，金属蛋白酶 ADAM 样 decysin 1 (ADAMDEC1) 在炎症反应的早期阶段发挥作用。我们研究了 ADAMDEC1 在 CRSwNP 发病机制中的作用。材料和方法：我们使用免疫组织化学和 RT-qPCR 比较了 CRS 患者鼻息肉组织中 ADAMDEC1 的表达。培养巨噬细胞，并在基线和暴露于脂多糖 (LPS) 后测定 ADAMDEC1 表达。结果：ADAMDEC1 在对照患者的组织中几乎检测不到，但与 ECRSwNP 组相比，在 NECRSwNP 组中表达较高。在鼻息肉组织中，ADAMDEC1由CD68阳性细胞表达，ADAMDEC1阳性细胞与CD68阳性细胞之间以及ADAMDEC1与CD68 mRNA水平之间呈正相关。此外，用LPS刺激单核细胞源性巨噬细胞诱导ADAMDEC1表达。结论和意义：本研究表明ADAMDEC1参与NECRSwNP的发病机制，也参与巨噬细胞中细菌内毒素信号传导。然而，其根本机制仍有待阐明。
Background: Chronic rhinosinusitis with nasal polyps (CRSwNP) is classified into two subtypes: eosinophilic (ECRSwNP) and non-eosinophilic (NECRSwNP). Although the inflammatory patterns of ECRSwNP have been elucidated, NECRSwNP is poorly understood.Aims/objectives: The metalloproteinase ADAM-like decysin 1 (ADAMDEC1) has been reported to play a role in the early stages of the inflammatory response. We investigated the role of ADAMDEC1 in the pathogenesis of CRSwNP.Material and methods: We compared ADAMDEC1 expression in nasal polyp tissue from CRS patients using immunohistochemistry and RT-qPCR. Macrophages were cultured and ADAMDEC1 expression was determined at baseline and after exposure to lipopolysaccharide (LPS).Results: ADAMDEC1 was virtually undetectable in tissues from control patients but was highly expressed in the NECRSwNP group compared with the ECRSwNP group. In nasal polyp tissues, ADAMDEC1 was expressed by CD68-positive cells, with a positive correlation between ADAMDEC1-positive and CD68-positive cells, and also between ADAMDEC1 and CD68 mRNA levels. Furthermore, stimulation of monocyte-derived macrophages with LPS induced ADAMDEC1 expression.Conclusions and significance: This study demonstrates that ADAMDEC1 is involved in the pathogenesis of NECRSwNP, and also bacterial endotoxin signalling in macrophages; however, the underlying mechanism remains to be elucidated.