Real-time measurements of endogenous CO production from vascular cells using an ultrasensitive laser sensor.
Real-time measurements of endogenous CO production from vascular cells using an ultrasensitive laser sensor.
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DOI：
10.1152/ajpheart.2001.280.1.h483
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
Yuji Morimoto;Yuji Morimoto;William Durante;D. G. Lancaster;D. G. Lancaster;J. Klattenhoff;F. Tittel
中科院分区：
文献类型：
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作者：
Yuji Morimoto;Yuji Morimoto;William Durante;D. G. Lancaster;D. G. Lancaster;J. Klattenhoff;F. Tittel
一氧化碳（CO）被认为是一种类似于一氧化氮的生物信使分子。基于中红外激光吸收光谱的紧凑型气体传感器被开发用于直接和实时测量血管细胞释放的痕量水平（约pmol）的CO。利用两束近红外激光在非线性光学晶体中的差频混频产生中红外光。选择CO的强红外吸收线（4.61 μ m），以便于CO检测，而不受其他气体种类的干扰。该传感器每隔20 s检测一次培养的血管平滑肌细胞产生的CO，灵敏度可达6.9pmol CO（0.25 nmol/10（7）细胞/h）、硝普钠处理的细胞（0.29 nmol/10（7）细胞/h）和氯化血红素处理的细胞（0.49 nmol/10（7）细胞/h）。该传感器还检测到，在加入血红素加氧酶（HO）抑制剂锡原卟啉-IX后，CO产生减少（从0.49 nmol/10（7）个细胞/h增加到0.02 nmol/10（7）个细胞/h），而在给予HO底物氯化血红素后，CO产生增加（从0.27 nmol/10（7）个细胞/h增加到0.64 nmol/10（7）个细胞/h）。这些结果表明，中红外激光吸收光谱是一种有用的技术，用于非侵入性和实时检测的痕量水平的CO从生物组织。
Carbon monoxide (CO) has been implicated as a biological messenger molecule analogous to nitric oxide. A compact gas sensor based on a midinfrared laser absorption spectroscopy was developed for direct and real-time measurement of trace levels (in approximate pmol) of CO release by vascular cells. The midinfrared light is generated by difference frequency mixing of two nearinfrared lasers in a nonlinear optical crystal. A strong infrared absorption line of CO (4.61 microm) is chosen for convenient CO detection without interference from other gas species. The generation of CO from cultured vascular smooth muscle cells was detected every 20 s without any chemical modification to the CO. The sensitivity of the sensor reached 6.9 pmol CO. CO synthesis was measured from untreated control cells (0.25 nmol per 10(7) cells/h), sodium nitroprusside-treated cells (0.29 nmol per 10(7) cells/h), and hemin-treated cells (0.49 nmol per 10(7) cells/h). The sensor also detected decreases in CO production after the addition of the heme oxygenase (HO) inhibitor tin protoporphyrin-IX (from 0.49 to 0.02 nmol per 10(7) cells/h) and increases after the administration of the HO substrate hemin (from 0.27 to 0.64 nmol per 10(7) cells/h). These results demonstrate that midinfrared laser absorption spectroscopy is a useful technique for the noninvasive and real-time detection of trace levels of CO from biological tissues.