Bead-based profiling of tyrosine kinase phosphorylation identifies SRC as a potential target for glioblastoma therapy.
Bead-based profiling of tyrosine kinase phosphorylation identifies SRC as a potential target for glioblastoma therapy.
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DOI：
10.1038/nbt.1513
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发表时间：
2009-01
影响因子：
46.9
通讯作者：
Golub, Todd R.
中科院分区：
文献类型：
--
作者：
Du, Jinyan;Bernasconi, Paula;Clauser, Karl R.;Mani, D. R.;Finn, Stephen P.;Beroukhim, Rameen;Burns, Melissa;Julian, Bina;Peng, Xiao P.;Hieronymus, Haley;Maglathlin, Rebecca L.;Lewis, Timothy A.;Liau, Linda M.;Nghiemphu, Phioanh;Mellinghoff, Ingo K.;Louis, David N.;Loda, Massimo;Carr, Steven A.;Kung, Andrew L.;Golub, Todd R.
作者贡献JD开发了Luminex免疫夹心法，用该方法描述了细胞系和原发胶质瘤，对SRC和达沙替尼进行了体外实验，准备了用于IP-MS分析的样品，并为手稿的规划和写作做出了贡献。PB为Luminex检测和IP-MS分析、Luminex检测抗体验证、SRC和Dasatinib的体外实验以及手稿的撰写做出了贡献。KRC和SAC进行了质谱分析。DM对Luminex试验进行变异分析。SPF和ML在组织芯片上进行IHC，并对染色结果进行分析。RB提供了原发胶质瘤样本的基因组数据。用Luminex比色法对细胞株进行亚甲基蓝染色筛选。BJ对抗体验证和手稿的撰写做出了贡献。XPP和HH为抗体验证提供了表达载体。RLM和TAL为本研究合成了达沙替尼。LML、PN和IKM提供了冷冻的原代胶质瘤标本和相应的基因组数据。DNL提供了原始的胶质瘤组织芯片。ALK对达沙替尼进行了动物实验。TRG计划和设计了该项目，并为手稿的规划和写作做出了贡献。
Authors contributions JD developed the Luminex immunosandwich assay, profiled cell lines and primary gliomas with the assay, performed the in vitro experiments on SRC and dasatinib, prepared samples for IP-MS analysis, and contributed to the planning and writing of the manuscript. PB contributed to sample preparation for the Luminex assay and IP-MS analysis, Luminex assay antibody validation, in vitro experiments on SRC and dasatinib, and writing the manuscript. KRC and SAC performed mass spectrometric analysis. DM performed the variation analysis on the Luminex assay. SPF and ML performed IHC on tissue microarrays and analyzed the staining results. RB provided the genomic data on the primary glioma samples. MB contributed to the cell line screen with the Luminex assay. BJ contributed to the antibody validation and writing of the manuscript. XPP and HH provided the expression constructs for antibody validation. RLM and TAL synthesized dasatinib for this study. LML, PN and IKM provided the frozen primary glioma specimens and corresponding genomic data. DNL provided the primary glioma tissue microarrays. ALK performed the animal experiments with dasatinib. TRG planned and designed the project and contributed to the planning and writing of the manuscript.
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DOI：
10.1073/pnas.0710052104
发表时间：
2007-12-11
影响因子：
11.1
作者：
Beroukhim, Rameen;Getz, Gad;Sellers, William R.
通讯作者：
Sellers, William R.
影响因子：
46.9
作者：
Rush, J;Moritz, A;Comb, MJ
通讯作者：
Comb, MJ
DOI：
10.1073/pnas.2235846100
发表时间：
2003-11-11
影响因子：
11.1
作者：
Rubin, JB;Kung, AL;Segal, RA
通讯作者：
Segal, RA
影响因子：
8
作者：
Tuveson, DA;Willis, NA;Demetri, GD
通讯作者：
Demetri, GD
影响因子：
64.8
作者：
HEISTERKAMP, N;STAM, K;GROSVELD, G
通讯作者：
GROSVELD, G