Metabolic Delivery of Methacryloyl Groups on Living Cells and Cell Surface Modification via Thiol-Ene "Click" Reaction
Metabolic Delivery of Methacryloyl Groups on Living Cells and Cell Surface Modification via Thiol-Ene "Click" Reaction
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DOI：
10.1002/mabi.201100242
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发表时间：
2011-11-10
影响因子：
4.6
通讯作者：
Matsuno, Hiroshi
中科院分区：
文献类型：
--
作者：
Iwasaki, Yasuhiko;Matsuno, Hiroshi
甲基丙烯酰通过糖基化途径传递到活细胞表面。甘露糖胺衍生物ManMA被合成为细胞表面唾液酸残基的前体。在含有ManMA的培养基中培养HeLa细胞，然后在光引发剂存在的情况下，将足量的PEG(4)10K-SH与细胞接触。然后将细胞暴露在紫外线下10分钟。荧光显微镜证实了PEG(4)10K-SH在细胞表面的固定作用，称为聚乙二醇化。表面修饰不影响细胞活力。细胞表面的生物素化也可以通过在聚乙二醇化过程中添加乙烯基化合物来实现。通过使用这个过程，细胞表面的功能可以自由控制。
Methacryloyl groups are delivered on a living cell surface via a glycosylation pathway. The mannosamine derivative ManMA is synthesized as a precursor of cell-surface sialic-acid residues. HeLa cells are cultivated in a culture medium containing ManMA, after which a sufficient amount of PEG(4)10K-SH is in contact with the cells in the presence of a photoinitiator. The cells are then exposed to UV-light for 10 min. The immobilization of PEG(4)10K-SH, termed PEGylation, on the cell surface is confirmed by fluorescence microscopy. The surface modification does not influence cell viability. Biotinylation of cell surface can also be achieved by the addition of a vinyl compound during PEGylation. By using this process, the functionalities of a cell surface can be freely controlled.