Association of serum interleukin-8 levels with the degree of fibrosis in infants with chronic liver disease
Association of serum interleukin-8 levels with the degree of fibrosis in infants with chronic liver disease
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DOI：
10.1097/00005176-200411000-00017
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发表时间：
2004-11-01
影响因子：
2.9
通讯作者：
De Benedetti, T
中科院分区：
文献类型：
--
作者：
Nobili, V;Marcellini, M;De Benedetti, T
目的：胆道闭锁是一种新生儿梗阻性胆道疾病，其特征是影响胆道树的肝内和肝外导管的破坏性、闭塞性过程，仅在生命的头几个月出现。进行性炎症和硬化反应的结果是梗阻性黄疸的发展。为了确定胆道闭锁儿童的促炎细胞因子谱，我们测量了循环中白细胞介素(IL)- i β、IL-6、肿瘤坏死因子α和IL-8的水平。方法：对12例胆道闭锁患儿（男5例，女7例）进行研究。此外，还包括4例进行性家族性肝内胆汁淤积症患者和3例Alagille综合征患者。5例新生儿肝炎患者作为无门脉纤维化的肝病对照进行了研究。在研究取样时，采用常规方法测定所有患者血清总胆红素和结合胆红素、γ -谷氨酰转移酶和谷丙转氨酶的浓度。肝活检的纤维化程度采用组织学活性指数评分。结果：在我们的研究中，12例胆道闭锁患者的I - I中检测到IL-8，中位水平为262 pg/ml，与对照组相比具有高度统计学意义（P < 0.0001）。进行性家族性肝内胆汁淤积症或Alagille综合征患者血清IL-8水平同样升高。在新生儿肝炎患者中，IL-8水平略有升高。血清IL-8水平与组织学活性指数显著相关（Rs, = 0.725, P < 0.0001）。结论：虽然需要进一步的研究来确定IL-8在门静脉炎症中的作用，但我们的研究结果表明，IL-8的产生增加可能是导致慢性肝病患者进行性门静脉炎症和纤维化的一个机制。
Objective: Biliary atresia is a neonatal obstructive cholangiopathy characterized by a destructive, obliterative process affecting both the intrahepatic and extrahepatic ducts of the biliary tree that uniquely presents in the first months of life. The consequence of progressive inflammatory and sclerotic reaction is the development of obstructive jaundice. To determine the proinflammatory cytokine profile in children with biliary atresia, we measured circulating levels of interleukin (IL)-Ibeta, IL-6, tumor necrosis factor-alpha and IL-8.Methods: Twelve children, five males and seven females, with biliary atresia were studied. In addition, four patients with progressive familial intrahepatic cholestasis and three with Alagille syndrome were also included. Five patients with neonatal hepatitis were studied as controls of a liver disease without portal fibrosis. Serum concentration of total and conjugated bilirubin, gamma-glutamyl transferase and glutamic-pyruvic transaminase were measured by routine methods in all patients at time of sampling for the study. The degree of fibrosis in liver biopsies was scored using the histologic activity index.Results: In our study IL-8 was detectable in I I of 12 patients with biliary atresia with a median level of 262 pg/ml and a highly statistically significant difference (P < 0.0001) from controls. In patients with progressive familial intrahepatic cholestasis or with Alagille syndrome serum IL-8 levels were similarly elevated. In patients with neonatal hepatitis, IL-8 levels were marginally increased. Serum IL-8 levels were significantly correlated (Rs, = 0.725, P < 0.0001) with the histologic activity index.Conclusions: Although further studies are needed to determine the role of IL-8 in portal inflammation, our results suggest that increased production of IL-8 may be a mechanism leading to the progressive portal inflammation and fibrosis in patients with chronic liver disease.