Clostridium ljungdahlii represents a microbial production platform based on syngas
Clostridium ljungdahlii represents a microbial production platform based on syngas
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DOI：
10.1073/pnas.1004716107
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发表时间：
2010-07-20
影响因子：
11.1
通讯作者：
Duerre, Peter
中科院分区：
文献类型：
--
作者：
Koepke, Michael;Held, Claudia;Duerre, Peter
扬氏梭菌是一种厌氧同型产乙酸菌，能够发酵糖、其他有机化合物或CO2/H-2和合成气（CO/H-2）。后一个特征使其成为生物技术行业的一种有趣的微生物，因为重要的大宗化学品和蛋白质可以以二氧化碳为代价生产，从而将工业需求与持续减少大气中的CO和CO2结合起来。对C. ljungdahlii揭示其包含4，630，065 bp，并且是迄今已知的最大梭菌基因组之一。实验数据和计算机模拟比较揭示了厌氧同型产乙酸代谢的第三种模式。与其他生物如Moorella thermoacetica或Acetobacterium woodii不同，细胞色素和钠离子都不参与能量产生。相反，存在Rnf系统，通过该系统可以进行质子易位。已经开发了一种电穿孔方法，用携带异源基因的质粒转化生物体用于丁醇生产。可以证明这些基因的成功表达，从而形成生物燃料。因此，C. ljungdahlii可用作基于合成气和二氧化碳/氢气混合物的独特微生物生产平台。
Clostridium ljungdahlii is an anaerobic homoacetogen, able to ferment sugars, other organic compounds, or CO2/H-2 and synthesis gas (CO/H-2). The latter feature makes it an interesting microbe for the biotech industry, as important bulk chemicals and proteins can be produced at the expense of CO2, thus combining industrial needs with sustained reduction of CO and CO2 in the atmosphere. Sequencing the complete genome of C. ljungdahlii revealed that it comprises 4,630,065 bp and is one of the largest clostridial genomes known to date. Experimental data and in silico comparisons revealed a third mode of anaerobic homoacetogenic metabolism. Unlike other organisms such as Moorella thermoacetica or Acetobacterium woodii, neither cytochromes nor sodium ions are involved in energy generation. Instead, an Rnf system is present, by which proton translocation can be performed. An electroporation procedure has been developed to transform the organism with plasmids bearing heterologous genes for butanol production. Successful expression of these genes could be demonstrated, leading to formation of the biofuel. Thus, C. ljungdahlii can be used as a unique microbial production platform based on synthesis gas and carbon dioxide/hydrogen mixtures.