Hypermethylation of CpGs in the promoter of the COL1A1 gene in the aged periodontal ligament
Hypermethylation of CpGs in the promoter of the COL1A1 gene in the aged periodontal ligament
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DOI：
10.1177/154405910608500308
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发表时间：
2006-03-01
影响因子：
7.6
通讯作者：
Ono, T
中科院分区：
文献类型：
--
作者：
Ohi, T;Uehara, Y;Ono, T
虽然人的牙周膜表现出与年龄相关的组织学变化，但其分子机制尚不清楚。我们先前发现COL1A1基因的表达随着年龄的增长而下降。在这项研究中，我们询问该基因调节区的DNA甲基化是否在衰老过程中发生变化，作为下降的可能原因。使用的方法是胞嘧啶的亚硫酸氢盐修饰和DNA的核苷酸测序。虽然第一内含子区域在年轻和老年时保持去甲基化状态，但启动子近端和远端的大多数CpG位点的甲基化水平随着年龄的增加而升高。对数据的分析表明，启动子区域的DNA超甲基化可能对COL1A1基因表达的年龄相关性降低具有重要意义。
Although the human periodontal ligament shows age-associated histological alterations, the molecular mechanisms are not yet understood. We previously found that COL1A1 gene expression declines with age. In this study, we asked whether DNA methylation in the regulatory region of the gene alters in the aging process, as a possible cause of the decline. The method used was a bisulfite modification of cytosine and nucleotide sequencing of DNA. While the 1st intron region was kept demethylated at young and old ages, the levels of methylation at most CpG sites in the proximal and distal regions of the promoter showed elevation at older ages. Analysis of the data indicates the possible importance of DNA hypermethylation in the promoter region for the age-associated decrease of COL1A1 gene expression.