ENERGY TRANSFER IN A BIOLUMINESCNET SYSTEM
ENERGY TRANSFER IN A BIOLUMINESCNET SYSTEM
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DOI：
10.1002/jcp.1040770305
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发表时间：
1971-01-01
影响因子：
5.6
通讯作者：
HASTINGS, JW
中科院分区：
文献类型：
--
作者：
MORIN, JG;HASTINGS, JW
腔肠动物中的许多（但不是全部）生物发光系统涉及从钙激活光蛋白的激发产物分子到第二种物质（绿色荧光蛋白）的能量转移，其激发态发射波长为 508 nm。尽管研究的所有发光腔肠动物都具有发光蛋白，但并非所有都具有绿色荧光蛋白。这种绿色荧光分子位于发光细胞中；因此可以通过荧光显微镜轻松区分它们。活性成分存在于亚细胞颗粒中；这些已通过在等渗（海水）盐溶液中均质化而以活性形式分离出来。
Many (but not all) of the bioluminescent systems in coelenter‐ates involve energy transfer from an excited product molecule of the calcium activated photoprotein to a second species, the green fluorescent protein, with emission at 508 nm from its excited state. Although all the luminescent coelen‐terates studied possess photoproteins, not all of them have the green fluorescent protein. This green fluorescent molecule is localized in the luminescent cells; they can thus be easily distinguished by fluorescence microscopy. The active components occur in subcellular particles; these have been isolated in an active form by homogenization in isotonic (to sea water) salt solutions.