Gut microbiota differences in Island Hispanic Puerto Ricans and mainland non-Hispanic whites during chemoradiation for rectal cancer: A pilot study
Gut microbiota differences in Island Hispanic Puerto Ricans and mainland non-Hispanic whites during chemoradiation for rectal cancer: A pilot study
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DOI：
10.1016/j.currproblcancer.2020.100551
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发表时间：
2020-08-01
影响因子：
2.6
通讯作者：
Colon-Otero, Gerardo
中科院分区：
文献类型：
--
作者：
Gonzalez-Mercado, Velda J.;Lim, Jean;Colon-Otero, Gerardo
目的：调查直肠癌放化疗 (CRT) 之前和结束时测量的岛屿西班牙裔波多黎各人 (HPR) 和大陆非西班牙裔白人 (NHW) 肠道微生物组的多样性、分类组成和预测功能途径是否存在差异。方法：对新诊断直肠癌患者的 56 份粪便样本（25 名 HPR 和 31 NHW）进行了研究。放化疗期间进行扩增子测序。使用 QIIME2、phyloseq 和 LEfSe 分析 16S rRNA 基因数据。结果：我们在 CRT 期间观察到 HPR 和 NHW 参与者的样本内 α 多样性相似。然而，在 CRT 结束时，两个组中的几个类群的丰度存在显着差异。与 NHW 相比，HPR 肠道中富集的类群包括 Muribaculaceae、Prevotella 2 和 7、Gemella、Bacillales Family XI、Catenibacter、Sutterella、Pasteurellales 和 Pasteurellaceae 属，而 NHW 参与者中过多的类群是 Turicibacter 和 Eubacteriaceae。预测的 HPR 微生物群功能的显着差异包括 L-甲硫氨酸的合成以及苯乙胺和苯乙酸的降解途径。结论：在这项初步研究中，肠道微生物组的分类分析和功能预测表明，与 NHW 参与者相比，接受 CRT 的 HPR 直肠癌患者的肠道微生物功能具有更大的炎症潜力。 (C) 2020 Elsevier Inc. 保留所有权利。
Purpose: To investigate whether there are differences in diversity, taxonomic composition, and predicted functional pathways of the gut microbiome between Island Hispanic Puerto Ricans (HPR) and mainland non-Hispanic whites (NHW) measured before and at the end of chemo-radiation (CRT) for Rectal Cancer.Methods: Fifty-six stool samples of newly diagnosed rectal cancer patients (25 HPR and 31 NHW) were amplicon-sequenced during chemo-radiotherapy. 16S rRNA gene data was analyzed using QIIME2, phyloseq, and LEfSe.Results: We observed similar within-sample alpha diversity for HPR and NHW participants during CRT. However, at the end of CRT, several taxa were present at significantly different abundances across both groups. Taxa enriched in the gut of HPR compared to NHW included Muribaculaceae, Prevotella 2 and 7, Gemella, Bacillales Family XI, Catenibacterium, Sutterella, Pasteurellales, and Pasteurellaceae genera, whereas over-represented taxa in NHW participants were Turicibacter and Eubacteriaceae. Significant differences in predicted HPR microbiota functions included pathways for synthesis of L-methionine and degradation of phenylethylamine and phenylacetate.Conclusion: In this pilot study, taxonomic analyses and functional predictions of the gut microbiomes suggest greater inflammatory potential in gut microbial functions among HPR rectal cancer patients undergoing CRT compared to that of NHW participants. (C) 2020 Elsevier Inc. All rights reserved.