Interferon regulatory factor-2 induces megakaryopoiesis in mouse bone marrow hematopoietic cells
Interferon regulatory factor-2 induces megakaryopoiesis in mouse bone marrow hematopoietic cells
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DOI：
10.1016/j.febslet.2009.10.006
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发表时间：
2009-11-03
期刊：
影响因子：
3.5
通讯作者：
Yamaguchi, Kazunari
中科院分区：
文献类型：
--
作者：
Masumi, Atsuko;Hamaguchi, Isao;Yamaguchi, Kazunari
巨核细胞的生成与IN有关。炎性反应。为探讨干扰素调节因子(IRFs)在炎症相关巨核细胞生成中的作用，对小鼠骨髓造血干细胞(HSCs)进行分析。干扰素-γ诱导HSC表达IRF-2以及CD41和IRF-1。体外克隆形成实验表明，过表达IRF-2而不是IRF-1的细胞能增加巨核细胞集落的数量。IRF-2基因表达上调造血细胞CD41启动子活性。在注射表达IRF-2的骨髓细胞的小鼠中，CD41阳性的骨髓细胞数量增加。这些发现提示，IRF-2在IN的巨核细胞生成中起重要作用。不稳定的状态。(C)2009年欧洲生化学会联合会。爱思唯尔出版公司版权所有。
Megakaryopoiesis is associated with in. ammatory reactions. To investigate the role of interferon regulatory factors (IRFs) in inflammation-associated megakaryopoiesis, mouse bone marrow hematopoietic stem cells (HSCs) were analyzed. IFN-gamma treatment induced IRF-2 expression as well as the expression of CD41 and IRF-1 in HSCs. An in vitro clonogenic assay showed that IRF-2- but not IRF-1-overexpressing cells increased the number of megakaryocytic colonies. IRF-2 transfection up-regulated CD41 promoter activity in hematopoietic cell lines. The number of CD41-positive bone marrow cells increased in mice injected with IRF-2-expressing bone marrow cells. These findings suggest that IRF-2 plays an important role in megakaryopoiesis in in. ammatory states. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.