High-resolution imaging of normal anatomy, and neural and adrenal malformations in mouse embryos using magnetic resonance microscopy
High-resolution imaging of normal anatomy, and neural and adrenal malformations in mouse embryos using magnetic resonance microscopy
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DOI：
10.1046/j.1469-7580.2003.00157.x
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发表时间：
2003-02-01
影响因子：
2.4
通讯作者：
Bhattacharya, S
中科院分区：
文献类型：
--
作者：
Schneider, JE;Bamforth, SD;Bhattacharya, S
基因或环境操作对小鼠发育影响的有效研究依赖于对大量胚胎先天畸形的快速准确筛查。在这里，我们证明，通过磁共振显微镜可以在比传统组织学短得多的时间内检查正常器官发育并识别小鼠胚胎的畸形。我们在不到 9 小时的过夜运行中对胚胎进行成像，操作时间不到 1 小时。在正常胚胎中，我们观察到了大脑、脊髓、神经节、眼睛、内耳、垂体、甲状腺、胸腺、气管、支气管、肺、心脏、肾脏、性腺、肾上腺、食道、胃、肠、脾、肝和胰腺。对缺乏转录共激活因子 Cited2 的胚胎进行的大脑检查显示，除了外脑畸形外，还存在小脑和中脑顶部发育不全的情况。在这些胚胎中，我们还能够检测到肾上腺发育不全。我们通过组织学切片证实了所有畸形。因此，磁共振显微镜可用于快速鉴定通过转基因技术产生的突变小鼠胚胎中的发育和器官畸形，在高通量诱变筛选中，或在筛选中识别致畸化合物和导致发育畸形的环境因素。
An efficient investigation of the effects of genetic or environmental manipulation on mouse development relies on the rapid and accurate screening of a substantial number of embryos for congenital malformations. Here we demonstrate that it is possible to examine normal organ development and identify malformations in mouse embryos by magnetic resonance microscopy in a substantially shorter time than by conventional histology. We imaged embryos in overnight runs of under 9 h, with an operator time of less than 1 h. In normal embryos we visualized the brain, spinal cord, ganglia, eyes, inner ear, pituitary, thyroid, thymus, trachea, bronchi, lungs, heart, kidneys, gonads, adrenals, oesophagus, stomach, intestines, spleen, liver and pancreas. Examination of the brain in embryos lacking the transcriptional coactivator Cited2 showed cerebellar and midbrain roof agenesis, in addition to exencephaly. In these embryos we were also able to detect agenesis of the adrenal gland. We confirmed all malformations by histological sectioning. Thus magnetic resonance microscopy can be used to rapidly identify developmental and organ malformations in mutant mouse embryos generated by transgenic techniques, in high-throughput mutagenesis screens, or in screens to identify teratogenic compounds and environmental factors contributing to developmental malformations.