T-CELL CLONING TO DETECT THE MUTANT 6-THIOGUANINE-RESISTANT LYMPHOCYTES PRESENT IN HUMAN PERIPHERAL-BLOOD
T-CELL CLONING TO DETECT THE MUTANT 6-THIOGUANINE-RESISTANT LYMPHOCYTES PRESENT IN HUMAN PERIPHERAL-BLOOD
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DOI：
10.1073/pnas.79.21.6617
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
BORCHERDING, WR
中科院分区：
文献类型：
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作者：
ALBERTINI, RJ;CASTLE, KL;BORCHERDING, WR
Rare thioguanine-resistant T lymphocytes, present in vivo in human peripheral blood, were isolated and grown in vitro as thioguanine-resistant cultured T cells. The conditions for their selection in vitro were such that thioguanine resistant had to have arisen in vivo. The mutant cells bore T-cell surface markers, maintained their thioguanine resistance in vitro in the presence or absence of selection, and were deficient in hypoxanthine-guanine phosphohribosyltransferase activity.
