Gut microbiome composition and risk factors in a large cross-sectional IBS cohort
Gut microbiome composition and risk factors in a large cross-sectional IBS cohort
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DOI：
10.1136/bmjgast-2019-000345
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发表时间：
2020-01-01
影响因子：
3.1
通讯作者：
Hoaglin, Michael C.
中科院分区：
文献类型：
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作者：
Agnello, Melissa;Carroll, Lauren N.;Hoaglin, Michael C.
目的肠易激综合征（Irritable bowel syndrome， IBS）是一种常见的胃肠道疾病，由于其固有的异质性和病因不明，难以诊断和治疗。虽然有证据表明微生物组在肠易激综合征中的重要性，但这种关联仍然不明确。在当前的研究中，我们的目标是在微生物组组成、人口统计学和危险因素方面对自我报告的肠易激综合征患者的大型横断面队列进行表征。设计先前提交粪便样本进行16S微生物组测序的个体被发送一份关于IBS诊断、人口统计学、健康史、合并症、家族史和症状的综合调查。在报告诊断为IBS且没有任何合并症的个体和报告没有健康状况的个体之间比较对数比率转换的微生物类群丰度。单变量检验之后是控制相关混杂因素的多变量逻辑回归模型。结果在6386名受访者中，1692名报告了IBS的诊断，没有合并症，1124名报告没有健康状况。在调整混杂因素后，我们确定了3门，15属和19种与IBS显著相关。人口统计学风险因素包括肠道疾病家族史和去年报告使用抗生素。结论本研究结果在一个大队列中证实了IBS的重要危险因素，并支持肠道微生物组成变化与IBS发病机制的联系。研究结果还表明，监测和调查肠易激综合征患者的微生物组具有临床意义。此外，本文所描述的探索性模型为今后的研究奠定了基础。
Objective Irritable bowel syndrome (IBS) is a common gastrointestinal disorder that is difficult to diagnose and treat due to its inherent heterogeneity and unclear aetiology. Although there is evidence suggesting the importance of the microbiome in IBS, this association remains poorly defined. In the current study, we aimed to characterise a large cross-sectional cohort of patients with self-reported IBS in terms of microbiome composition, demographics, and risk factors.Design Individuals who had previously submitted a stool sample for 16S microbiome sequencing were sent a comprehensive survey regarding IBS diagnosis, demographics, health history, comorbidities, family history, and symptoms. Log ratio-transformed abundances of microbial taxa were compared between individuals reporting a diagnosis of IBS without any comorbidities and individuals reporting no health conditions. Univariable testing was followed by a multivariable logistic regression model controlling for relevant confounders.Results Out of 6386 respondents, 1692 reported a diagnosis of IBS without comorbidities and 1124 reported no health conditions. We identified 3 phyla, 15 genera, and 19 species as significantly associated with IBS after adjustment for confounding factors. Demographic risk factors include a family history of gut disorders and reported use of antibiotics in the last year.Conclusion The results of this study confirm important IBS risk factors in a large cohort and support a connection for microbiome compositional changes in IBS pathogenesis. The results also suggest clinical relevance in monitoring and investigating the microbiome in patients with IBS. Further, the exploratory models described here provide a foundation for future studies.