Takifugu obscurus is a euryhaline fugu species very close to Takifugu rubripes and suitable for studying osmoregulation.
Takifugu obscurus is a euryhaline fugu species very close to Takifugu rubripes and suitable for studying osmoregulation.
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DOI：
10.1186/1472-6793-5-18
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发表时间：
2005-12-20
期刊：
影响因子：
--
通讯作者：
Hirose S
中科院分区：
文献类型：
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作者：
Kato A;Doi H;Nakada T;Sakai H;Hirose S
红鳍东方鲀的基因组序列是鱼类分子生理学研究中非常有用的工具。广盐性鱼类既能在淡水中生存，也能在海水中生存，是研究鱼类生理，特别是摄食调节的重要材料。最近我们了解到，有一种河豚，暗纹东方鲀，俗称“mefugu”，迁移到FW产卵。如果T. obscurus是一种广盐性鱼类，与T. rubripes，将成为研究红鳍金枪鱼生长调节机制的一种上级动物模型。因此，我们确定了它的广盐性和系统发育的关系，以东方家庭的成员。以下六种东方鲀用于分析：obscurus，T. rubripes，红柄T. niphobles，T. pardalis，T. poecilonotus和T.卟啉。当转移到FW时，只有T。在FW条件下，暗纹夜蛾能存活10天以上，而其它几种不能存活10天以上。在FW适应过程中，血清Na+浓度显著降低。obscurus仅轻微下降，但即使在T. niphobles，一种常见于咸淡水河口的边缘淡水物种。利用线粒体16S核糖体RNA基因核苷酸序列进行系统发育分析表明，这6种东方鲀属鱼类之间的亲缘关系非常近。T.暗纹东方鲀的基因组序列与其它东方鲀的基因组序列具有很高的同源性，可以利用现有的东方鲀基因组信息资源。这些特性使得在本研究之前很少引起动物生理学家关注的“河豚”成为研究维持体液稳态的分子机制的有用模型动物。
The genome sequence of the pufferfish Takifugu rubripes is an enormously useful tool in the molecular physiology of fish. Euryhaline fish that can survive both in freshwater (FW) and seawater (SW) are also very useful for studying fish physiology, especially osmoregulation. Recently we learned that there is a pufferfish, Takifugu obscurus, common name "mefugu" that migrates into FW to spawn. If T. obscurus is indeed a euryhaline fish and shares a high sequence homology with T. rubripes, it will become a superior animal model for studying the mechanism of osmoregulation. We have therefore determined its euryhalinity and phylogenetic relationship to the members of the Takifugu family. The following six Takifugu species were used for the analyses: T. obscurus, T. rubripes, T. niphobles, T. pardalis, T. poecilonotus, and T. porphyreus. When transferred to FW, only T. obscurus could survive while the others could not survive more than ten days in FW. During this course of FW adaptation, serum Na+ concentration of T. obscurus decreased only slightly, but a rapid and large decrease occurred even in the case of T. niphobles, a peripheral fresh water species that is often seen in brackish river mouths. Phylogenetic analysis using nucleotide sequences of the mitochondrial 16S ribosomal RNA gene of each species indicated that the six Takifugu species are very closely related with each other. T. obscurus is capable of adapting to both FW and SW. Its genomic sequence shares a very high homology with those of the other Takifugu species such that the existing Takifugu genomic information resources can be utilized. These properties make "mefugu", which has drawn little attention from animal physiologists until this study, a useful model animal for studying the molecular mechanism of maintaining body fluid homeostasis.