Synthesis of gold-cellobiose nanocomposites for colorimetric measurement of cellobiase activity
Synthesis of gold-cellobiose nanocomposites for colorimetric measurement of cellobiase activity
复制标题
用于纤维二糖酶活性比色测量的金-纤维二糖纳米复合材料的合成
DOI：
10.1016/j.saa.2014.04.091
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发表时间：
2014-11-11
影响因子：
4.4
通讯作者：
He, Yibin
中科院分区：
文献类型：
--
作者：
Lai, Cui;Zeng, Guang-Ming;He, Yibin
Gold-cellobiose nanocomposites (GCNCs) were synthesized by reducing gold salt with a polysaccharide, cellobiose. Here, cellobiose acted as a controller of nucleation or stabilizer in the formation of gold nano-particles. The obtained GCNCs were characterized with UV-visible spectroscopy; Zetasizer and Fourier transform infrared (FT-IR) spectrophotometer. Moreover, 6-Mercapto-1-hexanol (MCH) was modified on GCNCs, and the MCH-GCNCs were used to determine the cellobiase activity in compost extracts based on the surface plasmon resonance (SPR) property of MCH-GCNCs. The degradation of cellobiose on MCH-GCNCs by cellobiase could induce the aggregation, and the SPR absorption wavelength of MCH-GCNCs correspondingly red shifted. Thus, the absorbance ratio of treated MCH-GCNCs (A650/A520) could be used to estimate the cellobiase activity, and the probe exhibited highly sensitive and selective detection of the cellobiase activity with a wide linear from 3.0 to 100.0 U L-1 within 20 min. Meanwhile, a good linear relationship with correlation coefficient of R-2 = 0.9976 was obtained. This approach successfully showed the suitability of gold nanocomposites as a colorimetric sensor for the sensitive and specific enzyme activity detection. (C) 2014 Elsevier B.V. All rights reserved.
