Fibrillary co-deposition of laminin-5 and large unspliced tenascin-C in the invasive front of oral squamous cell carcinoma in vivo and in vitro
Fibrillary co-deposition of laminin-5 and large unspliced tenascin-C in the invasive front of oral squamous cell carcinoma in vivo and in vitro
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DOI：
10.1007/s004320000205
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发表时间：
2001-05-01
影响因子：
3.6
通讯作者：
Kosmehl, H
中科院分区：
文献类型：
--
作者：
Berndt, A;Borsi, L;Kosmehl, H
目的：口腔鳞状细胞癌 (OSCC) 的侵袭与层粘连蛋白-5 (Ln-5) 合成、基底膜 (BM) 局部 Ln-5 丢失以及侵袭癌细胞复合物下方基质中的 Ln-5 沉积有关。方法：本研究重点关注基底膜区域外 OSCC 侵袭前沿基质内的层粘连蛋白 5 基质重组，以及使用共焦激光扫描显微镜与未剪接腱蛋白-C (Tn-C-L) 和 ED-B+ 纤连蛋白 (ED-B+ fn) 相关的 OSCC-成纤维细胞共培养。结果：体内 Ln-5 被证明为侵入前沿的纤维沉积。它与 Tn-C-L 共定位。在纯 OSCC 培养物中，Ln-5 被合成并沉积为点状基质。未发现纤维结构。相反，在 OSCC-成纤维细胞共培养中，在 OSCC 细胞-成纤维细胞复合物界面内显示出纤维状 Ln-5 基质组织，仅与 Tn-C-L 和 ED-B+ fn 共分布。结论：至少在体外，癌细胞-基质成纤维细胞智能作用对于纤维化 Ln-5/Tn-C-L 基质组织是必不可少的。与器官型培养物中最初基底膜形成的相似之处在于，OSCC细胞-成纤维细胞界面处的纤维多蛋白复合物被认为是临时基底膜片段，可能对侵袭性肿瘤行为具有支持作用。
Purpose: Invasion of oral squamous cell carcinoma (OSCC) is associated with laminin-5 (Ln-5) synthesis, focal Ln-5 loss from the basement membrane (BM), and Ln-5 depositions in the stroma beneath invading carcinoma cell complexes. Methods: The study is focused on the laminin-5 matrix reorganisation within the stroma of the OSCC invasive front outside the basement membrane region as well as in OSCC-fibroblast co-culture in relation to unspliced tenascin-C (Tn-C-L) and ED-B+ fibronectin (ED-B+ fn) using confocal laser scanning microscopy. Resulrs: In vivo Ln-5 was demonstrated as fibrillary deposition in the invasive front. It was co-localised to Tn-C-L. In pure OSCC cultures, Ln-5 was synthesised and deposited as a spot-like matrix. Fibrillary structures were not found. In contrast, in the OSCC-fibroblast co-culture, a fibrillary Ln-5 matrix organisation was revealed within the interface of OSCC cell-fibroblast complexes exclusively in co-distribution with Tn-C-L and ED-B+ fn. Conclusion: At least in vitro, a carcinoma cell-stroma fibroblast intel action is indispensable for fibrillary Ln-5/Tn-C-L matrix organisation. Behind the parallels to the initial basement membrane formation in organotypic cultures, the fibrillary multiprotein complexes at the OSCC cell-fibroblast interface are suggested as provisional basement membrane fragments with a possible supportive role for invasive tumour behaviour.