Utility of an adenosine triphosphate bioluminescence assay to evaluate disinfection of Clostridium difficile isolation rooms.
Utility of an adenosine triphosphate bioluminescence assay to evaluate disinfection of Clostridium difficile isolation rooms.
复制标题
使用三磷酸腺苷生物发光测定来评估艰难梭菌隔离室的消毒。
DOI：
10.1086/671272
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发表时间：
2013
影响因子：
4.5
通讯作者：
Donskey,CurtisJ
中科院分区：
文献类型：
--
作者：
Deshpande,Abhishek;Sitzlar,Brett;Fertelli,Dennis;Kundrapu,Sirisha;Sunkesula,VenkataCK;Ray,AmyJ;Donskey,CurtisJ
艰难梭菌感染（CDI）患者出院后，必须对病房进行有效消毒，以防止传播。不幸的是，几项研究表明，环境培养对C保持阳性并不罕见。环境服务人员对CDI患者住院的房间（CDI房间）进行清洁和消毒后，培养C. difficile可能用于监测CDI房间的消毒，但它们既不广泛可用也不有效。需要一种易于使用和快速的方法来评估CDI房间消毒的有效性。三磷酸腺苷（ATP）生物发光检测可快速评估清洁效果，因为检测到表面上的ATP表明存在残留有机物质（例如细菌、人体分泌物或排泄物以及食物）。ATP的检测通常用于食品和饮料行业，并且越来越多地用于医疗保健设施，以评估清洁程序的充分性。目前尚不清楚表面上ATP的测量是否可用于评价CDI室的消毒。在这里，我们测试了CDI房间清洁表面上的低ATP读数预测C.很难
Effective disinfection of hospital rooms after discharge of patients with Clostridium difficile infection (CDI) is necessary to prevent transmission. Unfortunately, several studies have demonstrated that it is not uncommon for environmental cultures to remain positive for C. difficile after cleaning and disinfection of rooms in which a patient with CDI has been hospitalized (CDI rooms) by environmental services personnel. Cultures for C. difficile could potentially be useful to monitor disinfection of CDI rooms, but they are neither widely available nor efficient. There is a need for easy-to use and rapid methods to assess the effectiveness of CDI room disinfection. Adenosine triphosphate (ATP) bioluminescence assays provide a rapid assessment of cleaning effectiveness, because detection of ATP on surfaces indicates the presence of residual organic material (eg, bacteria, human secretions or excretions, and food). Detection of ATP is commonly used in the food and beverage industry and is increasingly being used in health care facilities to assess the adequacy of cleaning procedures. It is not known whether measurement of ATP on surfaces is useful to evaluate disinfection of CDI rooms. Here, we tested the hypothesis that low ATP readings on cleaned surfaces in CDI rooms would be predictive of negative cultures for C. difficile.