Enriched Environment Shortens the Duration of Action Potentials in Cerebellar Granule Cells
Enriched Environment Shortens the Duration of Action Potentials in Cerebellar Granule Cells
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DOI：
10.3389/fncel.2019.00289
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发表时间：
2019-07-16
影响因子：
5.3
通讯作者：
Hallermann, Stefan
中科院分区：
文献类型：
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作者：
Eshra, Abdelmoneim;Hirrlinger, Petra;Hallermann, Stefan
众所周知，啮齿动物的环境富集可以提高运动性能。结构和分子的变化已被报道与丰富的环境相结合，但单个神经元的功能改变仍然难以捉摸。在这里，我们比较了在对照条件下和丰富环境下饲养的小鼠。我们测试了旋转棒上的运动性能，随后进行了全细胞膜片钳记录小脑切片专注于颗粒细胞的小叶IX，这是已知的接收前庭输入。在丰富环境中饲养的小鼠能够在加速旋转棒上保持更长的时间。电生理分析显示颗粒细胞的正常被动特性和对丰富环境的功能适应，表现在更快的动作电位（AP）具有更高的去极化电压阈值和更大的AP过冲。此外，在丰富的环境中饲养的小鼠AP的最大放电频率更高。这些数据表明，丰富的环境导致神经元的生物物理特性的特定改变。此外，我们推测，小脑颗粒细胞产生更高的放电频率的能力，提高运动性能。
Environmental enrichment for rodents is known to enhance motor performance. Structural and molecular changes have been reported to be coupled with an enriched environment, but functional alterations of single neurons remain elusive. Here, we compared mice raised under control conditions and an enriched environment. We tested the motor performance on a rotarod and subsequently performed whole-cell patch-clamp recordings in cerebellar slices focusing on granule cells of lobule IX, which is known to receive vestibular input. Mice raised in an enriched environment were able to remain on an accelerating rotarod for a longer period of time. Electrophysiological analyses revealed normal passive properties of granule cells and a functional adaptation to the enriched environment, manifested in faster action potentials (APs) with a higher depolarized voltage threshold and larger AP overshoot. Furthermore, the maximal firing frequency of APs was higher in mice raised in an enriched environment. These data show that enriched environment causes specific alterations in the biophysical properties of neurons. Furthermore, we speculate that the ability of cerebellar granule cells to generate higher firing frequencies improves motor performance.