Epigenetic modulation on cat-cow interspecies somatic cell nuclear transfer embryos by treatment with trichostatin A.
Epigenetic modulation on cat-cow interspecies somatic cell nuclear transfer embryos by treatment with trichostatin A.
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DOI：
10.1111/asj.12676
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发表时间：
2017-04
期刊：
影响因子：
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通讯作者：
M. Wittayarat;Yoko Sato;L. Do;K. Chatdarong;T. Tharasanit;M. Techakumphu;M. Taniguchi;T. Otoi
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文献类型：
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作者：
M. Wittayarat;Yoko Sato;L. Do;K. Chatdarong;T. Tharasanit;M. Techakumphu;M. Taniguchi;T. Otoi
本研究旨在测定trichostatin A （TSA）处理的种间体细胞核移植（iSCNT）猫-牛（TSA）胚胎、未经TSA处理的iSCNT猫-牛对照（对照）胚胎和牛体外受精（IVF）胚胎早期胚胎发生过程中组蛋白H3 （H3K9ac/H3K18ac/H3K23ac; h3k9 /18/ 23ac）赖氨酸9/18/23位点的乙酰化和组蛋白H3 （H3K9me2）赖氨酸9位点的二甲基化，因为TSA-iSCNT胚胎可以发育成囊胚。与对照胚胎相比，在TSA-iSCNT胚胎和IVF胚胎的大多数后续阶段（融合/受精后2 h （PF/PI）至8细胞期），H3K9/18/23 ac的表达均较高。在PF/PI 6 h时，TSA-iSCNT胚胎和IVF胚胎中h3k9 / 23ac的表达低于对照胚胎，三组间H3K18ac的表达无差异。h3k9 / 23ac在原核（PN）期TSA-iSCNT胚胎和IVF胚胎中表达增加。H3K9me2在TSA-iSCNT胚胎中的表达与IVF胚胎在2 h PF/PI至PN期的表达相似，且表达水平高于对照胚胎。这些结果表明，用TSA处理iSCNT胚胎可以改变某些赖氨酸残基上的组蛋白修饰模式，其方式与在早期胚胎发生的IVF中所见的方式相当。
This study aimed to determine the acetylation at lysine 9/18/23 of histone H3 (H3K9ac/H3K18ac/H3K23ac; H3K9/18/23 ac) and the di-methylation at lysine 9 of histone H3 (H3K9me2) during early embryogenesis among trichostatin A (TSA)-treated interspecies somatic cell nuclear transfer (iSCNT) cat-cow (TSA-iSCNT) embryos, TSA-untreated iSCNT cat-cow control (control) embryos and bovine in vitro fertilization (IVF) embryos, because TSA-iSCNT embryos can develop to blastocysts. Compared to the control embryos, higher expressions of H3K9/18/23 ac were observed in TSA-iSCNT embryos and IVF embryos at most following stages (2 h post-fusion / post-insemination (PF/PI) to eight-cell stage). At 6 h PF/PI the expression of H3K9/23 ac in TSA-iSCNT embryos and IVF embryos were lower than those in control embryos, and the expression of H3K18ac was no difference among the three groups. The expression of H3K9/23 ac increased in TSA-iSCNT embryos and IVF embryos at pronuclear (PN) stages. The expression of H3K9me2 in TSA-iSCNT embryos resembled that of IVF embryos at 2 h PF/PI to PN stages, and these expression levels were greater than those of control embryos. These results suggest that treatment of iSCNT embryos with TSA modifies the patterns of histone modification at certain lysine residues in a manner that is comparable with that seen in IVF during early embryogenesis.