Radiosensitivity of mammalian cells. II. Radiation effects on macromolecular synthesis.
Radiosensitivity of mammalian cells. II. Radiation effects on macromolecular synthesis.
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哺乳动物细胞的放射敏感性。
DOI：
10.1016/s0006-3495(68)86568-3
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发表时间：
1968
影响因子：
3.4
通讯作者：
D. Petersen
中科院分区：
文献类型：
--
作者：
R. A. Walters;D. Petersen
研究了辐射对中国仓鼠细胞生命周期和分裂所必需的大分子合成的影响。采用大分子合成抑制剂的生命周期分析技术用于确定从辐射引起的分裂延迟自发恢复后特定群体的细胞生长动力学。结果表明，在缺乏功能性蛋白质合成的情况下，不会发生恢复。在抑制正常 RNA 和 DNA 合成的条件下，受辐射的细胞可以恢复生命周期和分裂的能力。还测量了编码辐射细胞分裂所必需的蛋白质的 mRNA 种类的稳定性。这些 mRNA 种类的平均功能寿命为 1 小时。数据表明存在一个由细胞组成的特定群体，这些细胞已完成与分裂相关的转录，但未完成翻译，并且可以从辐射损伤中恢复而无需合成额外的RNA。因此，分裂能力的最初恢复对蛋白质合成有一定的要求，但在原始信使保持完整的时期对核酸合成没有相应的要求。
Radiation effects on macromolecular synthesis essential for the Chinese hamster cell to traverse the life cycle and to divide have been investigated. Life-cycle analysis techniques employing inhibitors of macromolecular synthesis were used in determining the kinetics of cell growth for specific segments of the population following spontaneous recovery from radiation-induced division delay. The results indicated that recovery does not occur in the absence of functional protein synthesis. Under conditions which inhibit normal RNA and DNA synthesis, irradiated cells can recover the capacity to traverse the life cycle and to divide. The stability of mRNA species coding for proteins essential for division in irradiated cells was also measured. The mean functional lifetime of these mRNA species was 1 hr. The data demonstrate the existence of a specific segment of the population consisting of cells which have completed transcription related to division but not concomitant translation and which can recover from the radiation injury without synthesis of additional RNA. Thus, initial recovery of the ability to divide has an obligate requirement for protein synthesis but no corresponding requirement for nucleic acid synthesis during the period when original messenger remains intact.