A NEW HIGH-SENSITIVITY F-19 PROBE FOR LABELING CYSTEINE GROUPS OF PROTEINS
A NEW HIGH-SENSITIVITY F-19 PROBE FOR LABELING CYSTEINE GROUPS OF PROTEINS
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DOI：
10.1002/nbm.1940050605
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发表时间：
1992-11-01
影响因子：
2.9
通讯作者：
ZIMMERMANN, H
中科院分区：
文献类型：
--
作者：
KALBITZER, HR;ROHR, G;ZIMMERMANN, H
合成了一种新的碘乙酰胺标记物4-全氟叔丁基苯基碘乙酰胺（PFP）。它提供了均匀的F-19 NMR共振线，当与蛋白质偶联时可以高灵敏度检测。作为一个例子，肌动蛋白的巯基已经用PFP标记; < 100 nmol的这种中等大小的蛋白质（对应于2.5 mL的40 μ m溶液）可以在470 MHz的单次扫描中容易地检测到。
A novel iodoacetamide label 4-perfluoro-tert-butyl-phenyliodoacetamide (PFP) containing nine fluorine atoms in equivalent positions has been synthesized. It provides a homogeneous F-19 NMR resonance line which can be detected with high sensitivity when coupled to proteins. As an example, the sulfhydryl groups of actin have been labeled with PFP; < 100 nmol of this medium sized protein (corresponding to 2.5 mL of a 40mum solution) can be detected easily in a single scan at 470 MHz.