Contribution of horizontal gene transfer to the evolution of Saccharomyces cerevisiae
Contribution of horizontal gene transfer to the evolution of Saccharomyces cerevisiae
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DOI：
10.1128/ec.4.6.1102-1115.2005
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发表时间：
2005-06-01
期刊：
影响因子：
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通讯作者：
Dietrich, FS
中科院分区：
文献类型：
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作者：
Hall, C;Brachat, S;Dietrich, FS
半子囊菌酿酒酵母和阿什比亚棉霉的基因组已经完全测序，可以对这两个基因组进行比较分析，结果表明，由于来自细菌的水平基因转移，少数基因似乎已经进入这些基因组。在棉铃虫和酿酒酵母中分别鉴定出1例和10例水平基因转移的潜在病例，其中2例进行了进一步的研究。一个编码二氢罗酸脱氢酶(DHOD)的基因可能是水平基因转移的例子，从其他细菌和真菌物种中对该基因进行测序表明，该基因可以产生足够的数据来构建一个得到良好支持的系统发育图。在酿酒酵母中发现的DHOD编码基因URA1(YKL216W)似乎是在酿酒酵母谱系从白色念珠菌谱系分化后进入酵母科的，可能是因为从棉曲霉谱系分化而来的。这个基因似乎来自乳杆菌，在它获得后，内源真核细胞的DHOD基因丢失了。研究还表明，在酿酒酵母中，细菌水平转移的DHOD是厌氧合成尿嘧啶所必需的。另一个详细讨论的基因是BDS1，这是一种细菌来源的芳基和烷基硫酸酯酶基因，我们已经证明，它允许利用来自几个有机来源的硫酸盐。在真核生物中，该基因存在于酿酒酵母和贝亚氏酵母中，似乎来自于α-蛋白细菌。
The genomes of the hemiascomycetes Saccharomyces cerevisiae and Ashbya gossypii have been completely sequenced, allowing a comparative analysis of these two genomes, which reveals that a small number of genes appear to have entered these genomes as a result of horizontal gene transfer from bacterial sources. One potential case of horizontal gene transfer in A. gossypii and 10 potential cases in S. cerevisiae were identified, of which two were investigated further. One gene, encoding the enzyme dihydroorotate dehydrogenase (DHOD), is potentially a case of horizontal gene transfer, as shown by sequencing of this gene from additional bacterial and fungal species to generate sufficient data to construct a well-supported phylogeny. The DHOD-encoding gene found in S. cerevisiae, URA1 (YKL216W), appears to have entered the Saccharomycetaceae after the divergence of the S. cerevisiae lineage from the Candida albicans lineage and possibly since the divergence from the A. gossypii lineage. This gene appears to have come from the Lactobacillales, and following its acquisition the endogenous eukaryotic DHOD gene was lost. It was also shown that the bacterially derived horizontally transferred DHOD is required for anaerobic synthesis of uracil in S. cerevisiae. The other gene discussed in detail is BDS1, an aryl- and alkyl-sulfatase gene of bacterial origin that we have shown allows utilization of sulfate from several organic sources. Among the eukaryotes, this gene is found in S. cerevisiae and Saccharomyces bayanus and appears to derive from the alpha-proteobacteria.