Effect of copper ion on oxygen damage in superoxide dismutase-deficientSaccharomyces cerevisiae
Effect of copper ion on oxygen damage in superoxide dismutase-deficientSaccharomyces cerevisiae
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铜离子对超氧化物歧化酶缺陷型酿酒酵母氧损伤的影响
DOI：
10.1007/bf02976886
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发表时间：
1996
影响因子：
6.7
通讯作者：
K. Huh
中科院分区：
文献类型：
--
作者：
J. Lee;Ji M Kim;Su Won Kim;D. Nam;C. Yong;K. Huh
利用超氧化物歧化酶（SOD）缺陷的酿酒酵母（Saccharomycescerevisiae）突变株，研究了0.1mM铜离子（Cu ~（++））诱导的氧化胁迫。在有氧培养条件下，MnSOD（线粒体SOD）缺陷的酵母比CuZnSOD（细胞质SOD）缺陷的酵母表现出更明显的生长迟缓。然而，在厌氧条件下，这些突变体的生长模式与野生型相比没有太大差异。结果表明，在有氧培养条件下，添加0.1mM的Cu ~（++）能显著提高CuZnSOD的活性，但对MnSOD的活性影响不大。还观察到，过氧化氢酶活性似乎是相对较高的铜离子的存在下，尽管谷胱甘肽过氧化物酶的显着减少CuZnSOD缺陷的酵母，但过氧化氢酶和谷胱甘肽过氧化物酶的轻微增量检测MnSOD缺陷菌株。这表明，细胞质SOD的缺乏主要由过氧化氢酶来补偿。然而，这些现象导致在CuZnSOD缺陷的酵母细胞脂质过氧化物含量的显着增加和MnSOD缺陷的细胞脂质过氧化物略有增加。在添加铜离子的厌氧培养条件下，SOD缺陷型酵母细胞内过氧化氢酶和谷胱甘肽过氧化物酶的活性略有提高，而细胞膜脂质过氧化物的含量没有明显变化。这表明，铜离子在厌氧条件下产生的少量自由基可以被过氧化氢酶和谷胱甘肽过氧化物酶充分克服。
Using superoxide dismutase (SOD)-deficient mutants ofSaccharomyces cerevisiae, the oxidative stresses induced by 0.1 mM of copper ion (Cu++) was studied. In aerobic culture condition, yeasts lacking MnSOD (mitochondrial SOD) showed more significant growth retardation than CuZnSOD (cytoplasmic SOD)-deficient yeasts. However, not so big differences in growth pattern of those mutants compared with wild type were observed under anaerobic condition. It was found that, under aerobic condition, the supplementation of 0.1 mM copper ion (Cu++) into culture medium caused the remarkable increase of CuZnSOD but not so significant change in MnSOD. It was also observed that catalase activities appeared to be relatively high in the presence of copper ion in spite of the remarkable reduction of glutathion peroxidase in CuZnSOD-deficient yeasts, but the slight increments of catalase and glutathion peroxidase were detected in MnSOD-deficient strains. It implies that the lack of cytoplasmic SOD could be compensated mainly by catalase. However, these phenomena resulted in the significant increase of cellular lipid peroxides content in CuZnSOD-deficient yeasts and the slight increment of lipid peroxides in MnSOD-deficient cells. In anaerobic cultivation supplementing copper ion, the cellular enzyme activities of catalase and glutathion peroxidase in SOD-deficient yeasts were slightly increased without any significant changes of lipid peroxides in cell membrane. It suggests that a little amount of free radicals generated, by copper ion under anaerobic condition could be sufficiently overcome by catalase as well as glutathion peroxidase.
DOI：
10.1016/s0021-9258(19)36959-5
发表时间：
1992-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
X. Liu;I. Elashvili;E. B. Gralla;J. Valentine;P. Lapinskas;V. Culotta
通讯作者：
X. Liu;I. Elashvili;E. B. Gralla;J. Valentine;P. Lapinskas;V. Culotta
DOI：
10.1073/pnas.85.13.4789
发表时间：
1988-07-01
影响因子：
11.1
作者：
BERMINGHAMMCDONOGH, O;GRALLA, EB;VALENTINE, JS
通讯作者：
VALENTINE, JS